〜2000 2001 2002 2003 2004 2005 2006  
  2007 2008 2009 2010 2011 2012 2013
  2014  2015  2016  2017  2018 

英文原著論文(Original papers)

Suzuki K*, Onishi T*, Nakada C, Takei S, J. Daniels M, Nakano M, Matsuda T and Nagai T.
Uninterrupted monitoring of drug effects in human-induced pluripotent stem cell-derived cardiomyocytes with bioluminescence Ca2+ microscopy.
BMC Research Notes, in press (* equal contribution)

Riani Y D, Matsuda T, Takemoto K, Nagai T.
Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation.
BMC Biol, 16,1,2018

Hara-Kuge S, Nishihara T, Matsuda T, Kitazono T, Teramoto T, Nagai T and Ishihara T.
An improved inverse-type Ca2+ indicator can detect putative neuronal inhibition in Caenorhabditis elegans by increasing signal intensity upon Ca2+ decrease.
PLoS ONE, 13(4), e0194707,2018

Nadim H, Suzuki K, Iwano M, Matsuda T and Nagai T.
Bioluminescent low-affinity Ca2+ indicator for ER with multicolor calcium imaging in single living cells.
ACS Chemical Biology, in press

Ohta Y, Furuta T, Nagai T and Horikawa K.
Red fluorescent cAMP indicator with increased affinity and expanded dynamic range.
Sci. Rep., 8, 1866, 2018

Wazawa T, Arai Y, Kawahara Y, Takauchi H, Washio T, and Nagai T.
Highly biocompatible super-resolution fluorescence imaging using the fast photoswitching fluorescent protein Kohinoor and SPoD-ExPAN with Lp-regularized image reconstruction.
Microscopy, 67(2), 89-98, 2018

Kushida Y, Arai Y, Shimono K, and Nagai T.
Biomimetic chemical sensing by fluorescence signals using a virus-like particle-based platform.
ACS Sensors, 3(1), 87-92, 2018

Maeshima K*, Matsuda T*, Shindo Y*, Imamura H*, Tamura S, Imai R, Kawakami S, Nagashima R, Soga T, Noji H, Oka K and Nagai T.
A transient rise in free Mg2+ ions released from ATP-Mg by its hydrolysis contributes to mitotic chromosome condensation.
Current Biology, 28(3), 444-451.e6, 2018 (* equal contribution)

Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T.
Acid-tolerant monomeric GFP from Olindias formosa.
Cell Chemical Biology, 25(3), 330-338, 2018


Shinoda H, Michael S and Nagai T.
Fluorescent proteins for investigating biological events in acidic environments
International Journal of Molecular Science, Accepted


Nagai T, Riani YD.
Optogenetic control of generation of reactive oxygen species for photo-inducible protein inactivation and cell ablation.
Optogenetics, Royal Society of Chemistry Books, Accepted

Nagai T, Horikawa K, Saito K, Matsuda T.
Genetically encoded Ca2+ indicators; expanded affinity range, color hue and compatibility with optogenetics.
Application of Genetically Encoded Indicators to Mammalian Central Nervous System, Frontiers in Molecular Neuroscience, 38-42

英文原著論文(Original papers)

Toba S, Jin M, Yamada M, Kumamoto K, Matsumoto S, Yasunaga T, Fukunaga Y, Miyazawa A, Fujita S, Itoh K, Fushiki S, Hiroaki K, Wanibuchi H, Arai Y, Nagai T, and Hirotsune S.
Alpha-synuclein facilitates to form short unconventional microtubules that have a unique function in the axonal transport.
Sci. Rep., 7, 16386, 2017

Nozaki T, Imai R, Tanbo M, Nagashima R, Tamura S, Tani T, Joti Y, Tomita M, Hibino K, Kanemaki MT, Wendt KS, Okada Y, Nagai T, and Maeshima K.
Dynamic organization of chromatin domains revealed by superresolution live-cell imaging.
Molecular Cell, 67(2), 282-293, 2017

Aoyama M, *Yoshioka Y, Arai Y, Hirai H, Ishimoto R, Nagano K, Higashisaka K, Nagai T, *Tsutsumi Y.
Intracellular trafficking of particles inside endosomal vesicles is regulated by particle size.
Journal of Controlled Release, 260, 183-193, 2017

Seiriki K, Kasai A, Hashimoto T, Schulze W, Niu M, Yamaguchi S, Nakazawa T, Inoue K, Uezono S, Takada M, Naka Y, Igarashi H, Tanuma M, Waschek JA, Ago Y, Tanaka KF, Hayata-Takano A, Nagayasu K, Shintani N, Hashimoto R, Kunii Y, Hino M, Matsumoto J, Yabe H, Nagai T, Fujita K, Matsuda T, Takuma K, Baba A, and Hashimoto H.
High-speed and scalable whole-brain imaging in rodents and primates.
Neuron, 94(6), 1085-1100, 2017

Matsushita J*, Inagaki S*, Nishie T, Sakasai T, Tanaka J, Watanabe C, Mizutani K, Miwa Y, Matsumoto K, Takara K, Naito H, Kidoya H, Takakura N, Nagai T, Takahashi S and Ema M. (*: co-first author)
Fluorescence and bioluminescence imaging of angiogenesis in Flk1-Nano-lantern transgenic mice.
Sci. Rep., 7, 46597, 2017

Chang YF, Broyles CN, Brook F, Davies MJ, Turtle C, Nagai T and Daniels MJ.
Non-invasive phenotyping and drug testing in single cardiomyocytes or beta-cells by calcium imaging and optogenetics.
PLoS ONE, 12(4), e0174181, 2017

Nakano M, Arai Y, Kotera I, Okabe K, Kamei Y and Nagai T.
Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response
PLoS ONE, 12(2), e0172344, 2017

Suzuki M, Sato M, Koyama H, Hara Y, Hayashi K, Yasue N, Imamura H, Fujimori T, Nagai T, E. Campbell R, and Ueno N.
Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure
Development, 144, 1307-1316, 2017

Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G,J. Daniels M, Okamura Y, Matsuda T, and Nagai T.
Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging
Scientific Reports, 7, 42398, 2017

Takemoto K, Iwanari H, Tada H, Suyama K, Sano A, Nagai T, Hamakubo T, and Takahashi T.
Optical inactivation of synaptic AMPA receptors erases fear memory
Nature Biotechnology, 35, 38-47, 2017

Kim S, Arai Y, Tani T, Takatsuka H, Saito Y, Kawashima T, Kawakami S, Miyawaki A, and Nagai T.
Simultaneous imaging of multiple cellular events using high-accuracy fluorescence polarization microscopy.
Microscopy, 66(2), 110-119, 2017


The 31st Annual Conference of the Japanese Society for Artificial Intelligence, 2017

Inagaki S, and Nagai T.
Bioluminescent indicator applicable to membrane voltage recording in various excitable cell types.
SPIE Proceedings10251, 1025102, 2017


Suzuki K and Nagai T.
Recent progress in expanding the chemiluminescent toolbox for bioimaging.
Current Opinion in Biotechnology, 48, 135-141, 2017

Nakano M, Nagai T
Thermometers for monitoring cellular temperature.
Journal of Photochemistry and Photobiology, C: Photochemistry Reviews, 30, 2-9, 2017

Agetsuma M, Matsuda T, and Nagai T
Methods for monitoring signaling molecules in cellular compartments.
Cell Calcium, 64, 12-19, 2017



日本語総説(Reviews in Japanese)

実験医学, 35(19), 3184-3189, 2017

実験医学, 35(19), 3204-3210, 2017

生物物理, 57(5), 262-264, 2017

生体の科学, 68(5), 462-463, 2017

顕微鏡, 77-81, 2017

プレプリントサーバ (Preprint server)

Inagaki S, Agetsuma M, Ohara S, Iijima T, Wazawa T, Arai Y, Nagai T.
In vivo brain activity imaging of interactively locomoting mice
bioRxiv, Posted October 15, 2017

Arai Y, Takauchi H, Ogami Y, Fujiwara S, Nakano M, Nagai T.
A spontaneously blinking fluorescent protein for simple single laser super-resolution live cell imaging.
bioRxiv, Posted October 8, 2017

英文原著論文(Original papers)

Shimada N, Saito M, Shukuri S, Kuroyanagi S, Kuboki T, Kidoaki S, Nagai T, and Maruyama A.
Reversible monolayer/spheroid cell culture switching by UCST-type thermoresponsive ureido polymers.
ACS Applied Materials & Interfaces, 8(46), 31524 - 31529, 2016

Suzuki K, Kimura T, Shinoda H, Bai G, Daniels M, Arai Y, Nakano M, and Nagai T
Five color variants of bright luminescent protein for real-time multicolor bioimaging
Nature Communications, 7, 13718, 2016

Lu-Walther H, Hou W, Kielhorn M, Arai Y, Nagai T, Kessels M, Qualmann B, and Heintzmann R.
Nonlinear structured illumination using a fluorescent protein activating at the readout wavelength
PLoS ONE, 11(10), e0165148, 2016

Mochida S, Rata Scott, Hino H, Nagai T,and Novak B.
Two bistable swithes govern M phase entry
Current Biology, 26, 3361-3367, 2016

Sakamaki K, Ishii MT, Sakata T, Takemoto K, Takagi C, Takeuchi A, Morishita R, Takahashi H, Nozawa A, Shinoda H, Chiba K, Sugimoto H, Saito A, Tamate S, Satou Y, Sang-Kee J, Matsuoka S, Koyamada K, Sawasaki T, Nagai T,and Ueno N
Dysregulation of a potassium channel, THIK-1, targeted by caspase-8 accelerates cell shrinkage
Biochim. Biophys. Acta Mol. Cell Res. , 1863(11), 2766-2783, 2016

Ohta Y, Kamagata T, Mukai A, Takada S, Nagai T,and Horikawa K.
Non-trivial effect of the color-exchange of a Donor/Acceptor pair in the engineering of Forster resonance energy transfer (FRET)-based indicators.
ACS Chemical Biology, 11(7), 1816-1822, 2016

Pandey K, Ferreira PE, Ishikawa T, Nagai T, Kaneko O, and Yahata K
Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor
Scientific Reports 6, 23454, 2016

Lissek T, Obenhaus HA , Ditzel DAW, Nagai T, Miyawaki A , Sprengel R, and Hasan MT
General anesthetic conditions induce network synchrony and disrupt sensory processing in the cortex
Frontiers in Cellular Neuroscience , 4, 16, 2016

Morikawa TJ, Fujita H, Kitamura A, Horio T, Yamamoto J, Kinjo M, Sasaki A, Machiyama H, Yoshizawa K, Ichimura T, Imada K, Nagai T, and Watanabe TM.
Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it
Scientific Reports , 6, 22342, 2016


Inagaki S, and Nagai T
Current progress in genetically encoded voltage indicators for neural activity recording
Current Opinion In Chemical Biology , 33, 95-100, 2016

日本語総説(Reviews in Japanese)

Drug Delivery System, 31-2, 119-126, 2016

英文原著論文(Original papers)

Wazawa T, Morimoto, Nagai T. and Suzuki M.
Rotational motion of rhodamine 6G tethered to actin through oligo(ethylene glycol) linkers studied by frequency-domain fluorescence anisotropy
Biophysics and Physicobiology , 12, 87-102, 2017

Hanasaki I, Uehara S, Arai Y, Nagai T and Kawano S.
Threshold-free evaluation of near-surface diffusion and adsorption-dominated motion from single-molecule tracking data of single-stranded DNA through total internal reflection fluorescence microscopy
Jpn. J. Appl. Phys , 54(12), 125601, 2015

Iwano M, Ito K, Fujii S, Kakita M, Asano-Shimosato H, Igarashi M, Kaothien-Nakayama P, Entani T, Kanatani A, Takahisa M, Tanaka M, Komatsu K, Shiba H, Nagai T, Miyawaki A, Isogai A and Takayama S.
Calcium signalling mediates self-incompatibility response in the Brassicaceae
Nature Plants , 1, 15128, 2015

Kim K, Lakhanpal G, Lu HE, Khan M, Suzuki A, Kato-Hayashi M, Narayanan R, Luyben TT, Matsuda T, Nagai T, Blanpied TA, Hayashi Y, and Okamoto K.
A Temporary Gating of Actin Remodeling during Synaptic Plasticity Consists of the Interplay between the Kinase and Structural Functions of CaMKII
Neuron , 87(4), 813-826, 2015

Vadim PK, Matsuda T, Nagai T.
MagIC, a genetically encoded fluorescent indicator for monitoring cellular Mg2+ using a non-FRET ratiometric imaging approach
Journal of Biomedical Optics , 20(10), 101203, 2015

Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, Fujita K and Nagai T.
A fast- and positively photoswitchable fluorescent protein for ultralow-laser-power RESOLFT nanoscopy
Nature Methods, 12, 515-518, 2015

Nezu A, Morita T,Tojyo Y, Nagai T and Tanimura A.
Partial agonistic effects of pilocarpine on Ca2+ responses and salivary secretion in the submandibular glands of live animals
Experimental Physiology , 100, 640-651, 2015

Arai Y, Yamamoto T, Minamikawa T, Takamatsu T and Nagai T.
Spectral fingerprinting of individual cells visualized by cavity-reflection-enhanced light-absorption microscopy
PLoS ONE, 10, e0125733, 2015

Yamanaka M, Saito K, Smith IN, Arai Y, Uegaki K, Yonemaru Y, Mochizuki K, Kawata S, Nagai T and Fujita K
Visible-wavelength two-photon excitation microscopy for fluorescent protein imaging
Journal of Biomedical Optics, 20(10), 101202, 2015

Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin T, Okada Y and Nagai T.
Expanded palette of Nano-lantern for real-time muliti-color luminescence imaging
Proc. Natl. Acad. Sci. USA, 112, 4352-4356, 2015
(NHK 朝の関西ニュースにて放映、産経新聞・時事通信社・日刊工業新聞・日本経済新聞・毎日新聞に掲載)

Ichijo H, Hamada M, Takahashi S, Kobayashi M, Nagai T, Toyama T, Kawaguchi M.
Lateralization, maturation, and anteroposterior topography in the lateral habenula revealed by ZIF268/EGR1 immunoreactivity and labeling history of neuronal activity
Neuroscience Reserach, 95, 27-37, 2015

Sugiura K, Nagai T, Nakano M, Ichinose H, Nakabayashi T, Ohta N, Hisabori T.
Redox sensor proteins for highly sensitive direct imaging of intracellular redox state
Biochemical and Biophysical Research Communications, 457, 242-248, 2015


Uno S, Tiwari DK, Kamiya M, Arai Y, Nagai T, Urano Y.
A guide to use photocontrollable fluorescent proteins and synthetic smart fluorophores for nanoscopy.
Microscopy, 64, 4, 263-277, 2015

Saito K, Nagai T.
Recent progress in luminescent proteins development.
Current Opinion In Chemical Biology, 27, 46-51, 2015

Nagai T.
Real time imaging of biological phenomena with super-duper luminescent proteins
CYTOLOGIA, 80, 1-2, 2015

日本語総説(Reviews in Japanese)

肉眼でも観察できる! 3色(水色, 黄緑色, 橙色)の高光度発光タンパク質
O plus E, 428, 505-506, 2015

英文原著論文(Original papers)

Oshima Y, Imamura T, Shintani A, Kajiura-Kobayashi H, Hibi T, Nagai T, Nonaka S, Nemoto T.
Ultrasensitive imaging of Ca2+ dynamics in pancreatic acinar cells of Yellow Cameleon-Nano transgenic mice.
International Journal of Molecular Sciences, 15, 19971-19986, 2014

Yoshiyama S, Chen Z, Okagaki T, Kohama K, Nasu-Kawaharada R, Izumi T, Ohshima N, Nagai T, Nakamura A.
Nicotine exposure alters human vascular smooth muscle cell phenotype from a contractile to a synthetic type.
Atherosclerosis, 237, 464-470, 2014

Jin M, Yamada M, Arai Y, Nagai T, Hirotsune S.
Arl3 and LC8 regulate dissociation of dynactin from dynein.
Nature Communications,24, 5295, 2014

Kanemaru K, Sekiya H, Xu M, Satoh K, Kitajima N, Yoshida K, Okubo Y, Sasaki T, Moritoh S, Hasuwa H, Mimura M, Horikawa K, Matsui K, Nagai T, Iino M, Tanaka KF.
In vivo visualization of subtle, transient and local activity of astrocytes using an ultrasensitive Ca2+ indicator.
Cell Reports, 8, 311-318, 2014

Uehara S, Hanasaki I, Arai Y, Nagai T, Kawano S.
Statistical characterisation of single-stranded DNA motion near glass surface beyond diffusion coefficient.
Micro and Nano Letters, 9, 257?260, 2014

Fukuda N, Matsuda T, Nagai T.
Optical control of the Ca2+ concentration in a live specimen with a genetically encoded Ca2+-releasing molecular tool.
ACS Chemical biology, 9, 1197-1203, 2014


Nagai T, Horikawa K, Saito K, Matsuda T.
Genetically encoded Ca2+ indicators; expanded affinity range, color hue and compatibility with optogenetics.
Frontiers in Neuroscience, 7, 25, 2014

Arai Y, Nagai T.
Real-Time chemiluminescence imaging using Nano-Lantern probes.
Current Protocols in Chemical Biology, 6, 221-236

Matsuda T, Nagai T.
Quantitative measurement of intracellular protein dynamics using photobleaching or photoactivation of fluorescent proteins.
Microscopy, 63, 403-408, 2014

日本語総説(Reviews in Japanese)

化学, 69, 21-26, 2014

生体の科学, 65, 101-106, 2014

化学と生物, 52, 646-650, 2014

生物物理, 54, 75, 2014

化学フロンティア, 1分子ナノバイオ計測 15章

生化学, 86, 2014

英文原著論文(Original papers)

Takemoto K, Matsuda T, Sakai N, Fu D, Noda M, Uchiyama S, Kotera I, Arai Y, Horiuchi M, Fukui K, Ayabe T, Inagaki F, Suzuki H, Nagai T.
SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation.
Scientific Reports, 3, 2629, 2013

Nozaki T, Kaizu K, Pack C, Tamura S, Tani T, Hihara S, Nagai T, Takahashi K, Maeshima K.
Flexible and dynamic nucleosome fiber in living mammalian cells.
Nucleus, 4, 1?8, 2013

Yamanaka M, Saito K, Smith NI, Kawata S, Nagai T, Fujita K.
Saturated excitation (SAX) of fluorescent proteins for sub-diffraction-limited imaging of living cells in three dimensions.
Interface Focus, 3, 20130007, 2013

Yamada M, Kumamoto K, Mikuni S, Arai Y, Kinjo M, Nagai T, Tsukasaki Y, Watanabe TM, Fukui M, Jin M, Toba S, Hirotsune S.
Rab6a releases LIS1 from a dynein idling complex and activates dynein for retrograde movement.
Nature Communications, 4, 2033, 2013

Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang Y-F, Araki S, Arai Y, March K, Thomas H, Sagou K, Miyata T, Nagai T, Li W, Campbell R.
Improved orange and red Ca2+ indicators and photophysical considerations for optogenetic applications.
ACS Chemical Neuroscience, 4, 963?972, 2013

Matsuda T, Horikawa K, Saito K, Nagai T.
Highlighted Ca2+ imaging with a genetically encoded 'caged' indicator.
Scientific Reports, 3, 1398, 2013

Saito K, Higuchi Y, Arai Y, and Nagai T.
Video-rate imaging of luminescent tumour cells in freely moving unshaved mice.
Protocol Exchange, doi:10.1038/protex.2013.024

Hoi H, Matsuda T, Nagai T, Campbell RE.
Highlightable Ca2+ indicators for live cell imaging.
JACS, 135, 46?49, 2013


Arai.Y, and Nagai T.
Extensive use of FRET in biological imaging.
Microscopy, 62, 2013

Tiwari D.K., and Nagai T.
Smart fluorescent proteins: Innovation for barrier-free superresolution imaging in living cells.
Development Growth and Differentiation, 55, 491-507, 2013

Koldenkova V.P. and Nagai T.
Genetically encoded Ca2+ indicators: properties and evaluation.
BBA - Molecular Cell Research, 22, 2013

日本語総説(Reviews in Japanese)

顕微鏡, 48, 1-3, 2013

堀川 一樹、永井 健治
CLINICAL CALCIUM -循環器疾患とカルシウム・リン, 23, 2013


Hihara S, Pack CG, Hanafusa T, Kaizu K, Tani T, Takemoto S, Yoshimi T, Sako Y, Yokota H, Imamoto N, Kinjo M, Takahashi K, Nagai T, Maeshima K.
Local nucleosome dynamics facilitate chromatin accessibility in living mammalian cells.
Cell Reports, 2, 1645-56, 2012

Saito K, Chang Yu-Fen, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, Yoshida Y, Matsuda T, Arai Y, Nagai T.
Luminescent protein for high-speed single-cell and whole-body imaging.
Nature Communications, 3, 1262, 2012
Drug Discovery & Development誌にて当研究成果が紹介され、Nature Methods、
Nature Photonicsで紹介され、Faculty of 1000にノミネートされました。

Kominami K, Nagai T, Sawasaki T, Tsujimura Y,Yashima K, Sunaga Y, Tsuchimochi M, Nishimura J, Chiba K, Nakabayashi J, Koyamada K, Endo Y, Yokota H, Miyawaki A, Manabe N, Sakamaki K.
In vivo imaging of hierarchical spatiotemporal activation of caspase-8 during apoptosis.
PLoS ONE, 7, e50218, 2012

Hatsugai N, Koldenkova VP, Imamura H, Noji H, Nagai T.
Changes in cytosolic ATP levels and intracellular morphology during bacteria- induced hypersensitive cell death as revealed by real-time fluorescence microscopy imaging.
Plant and Cell Physiology, 53, 1768-1775, 2012

Iwano M, Ngo QA, Entani T, Shiba H, Nagai T, Miyawaki A, Isogai A,Grossniklaus U, Takayama S.
Cytoplasmic Ca2+ changes dynamically during the interaction of the pollen tube with synergid cells.
Development, 139, 4202-4209, 2012

Kwon HJ, Ohmiya Y, Honma K-i, Honma S,Nagai T,Saito K, Yasuda K.
Synchronized ATP oscillations have a critical role in prechondrogenic condensation during chondrogenesis.
Cell Death and Disease, 3, e278, 2012

Kominami K, Nakabayashi J, Nagai T, Tsujimura Y, Chiba K, Kimura H, Miyawaki A, Sawasaki T, Yokota H, Manabe N, Sakamaki K.
The molecular mechanism of apoptosis upon caspase-8 activation: Quantitative experimental validation of a mathematical model.
Biochim Biophys Acta-Molecular Cell Research, 1823, 1825?1840, 2012

Chang Y, Arai Y, Nagai T.
Optogenetic activation during detector 'dead time' enables compatible real-time fluorescence imaging.
Neuroscience Research, 73, 341?347, 2012

Ohtsuka K, Sato S, Sato Y, Sota K, Ohzawa S, Matsuda T, Takemoto K, Takamune N, Juskowiak B, Nagai T, Takenaka S T.
Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer?peptide conjugate.
Chem. Commun., 48, 4740?4742, 2012


おもろいバイオロジー ”蛍光プローブ開発秘話その2〜夏秋冬春〜”
細胞工学, 31, 2012


Saito K, Arai Y, Zhang J, Kobayashi K, Tani T, Nagai T.
Conjugation of both on-axis and off-axis light in Nipkow disk confocal microscope to increase availability of incoherent light source.
Cell Struct. Func. , 36, 237-246, 2011

Yamada Y, Michikawa T, Hashimoto M, Horikawa K, Nagai T, Miyawaki A,Hausser M, Mikoshiba K.
Quantitative comparison of genetically encoded Ca2+ indicators in cortical pyramidal cells and cerebellar Purkinje cells.
Frontiers in Cellular Neuroscience , 5, 18, 2011

Zhao Y, Araki S, Wu J, Teramoto T, Chang Y, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE.
An expanded palette of genetically encoded Ca2+ indicators.
Science , 333, 1888-1891, 2011

Nakano M, Imamura H, Nagai T, Noji H.
Ca2+ regulation of mitochondrial ATP synthesis visualized at the single cell level.
ACS Chemical Biology, 6, 709-15, 2011

Nomura M, Nagai T, Harada Y, Tani T.
Facilitated intracellular transport of TrkA by an interaction with nerve growth factor.
Developmental Neurobiology, 71, 634-649, 2011

Takemoto K, Matsuda T, McDougall M, Klaubert DH, Hasegawa A, Los GV, Wood KV, Miyawaki A, Nagai T.
Chromophore-assisted light inactivation of HaloTag fusion proteins labeled with eosin in living cells.
ACS Chemical Biology , 6, 401-406, 2011

Yang L, Matsuda T, Raviraj V, Ching YW, Braet F, Nagai T, Soon LL.
Imaging the dynamics of intracellular protein translocation by photoconversion of Phamret-Cybr/ROM.
Journal of Microscopy, 242, 250-261, 2011


生物物理, 52, 2011

CMC出版, 印刷中

生物の科学 , 遺伝 65, 87-91, 2011

化学と生物, 49, 555-559, 2011


Horikawa K, Yamada Y, Matsuda T, Kobayashi K, Hashimoto M, Matsu-ura T, Miyawaki A, Michikawa T, Mikoshiba K, Nagai T.
Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano.
Nature Methods, 7, 729-732, 2010

Saito K, Hatsugai N, Horikawa K, Kobayashi K, Matsu-Ura T, Mikoshiba K, Nagai T.
Auto-luminescent genetically-encoded ratiometric indicator for real-time Ca2+ imaging at the single cell level.
PLoS ONE, 5, e9935,2010

L?tcke H, Murayama M, Hahn T, Margolis DJ, Astori S, Borgloh SMzA, G?bel W, Yang Y, Tang W, K?gler S, Sprengel R, Nagai T, Miyawaki A, Larkum ME, Helmchen F, Hasan MT.
Optical recording of neuronal activity with a genetically-encoded Ca2+ indicator in anesthetized and freely moving mice.
Frontiers in Neural Circuit, 4, 1-12, 2010

Hong JH, Min CH, Jeong B, Kojiya T, Morioka E, Nagai T, Ikeda M. Lee K.
Intracellular calcium spikes in rat suprachiasmatic nucleus neurons induced by BAPTA-based calcium dyes.
PLoS ONE, 5, e9634,2010

Kotera I, Iwasaki T, Imamura H, Noji H, Nagai T.
Reversible dimerization of Aequorea victoria fluorescent proteins increases the dynamic range of FRET-based indicators.
ACS Chemical Biology, 5, 215-222, 2010


日本分子生物学会, 第十二章 153-167, 2010

1枚の写真館 幼名と改名
細胞工学, 29, 843, 2010

Dual FRET法を利用した複数の生理機能イメージング
シングルセル解析の最前線, 神原秀記, 松永是, 植田充美 監修 (分担執筆)
第一章 61-69, シーエムシー出版, 2010

レーザー研究, 38, 416-420, 2010

1波長励起Dual FRETによる生細胞内における複数現象の可視化
実験医学, 28, 589-598, 2010


Imamura H, Nhat KPH, Togawa H, Saito K, Iino R, Kato-Yamada Y, Nagai T, Noji H. 
Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators.
Proc. Natl, Aca, Sci. U.S.A., 106, 15651-15656, 2009

Iwano M, Entani T, Shiba H, Kakita H, Nagai T, Mizuno H, Miyawaki A, Shoji T, Kubo K, Isogai A, Takayama S.
Fine-tuning of the cytoplasmic Ca2+ concentration is essential for pollen tube growth.
Plant Physiology, 150, 1322-1334, 2009

Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa K, Nagai T.
An ultramarine fluorescent protein with increased photostability and pH insensitivity.
Nature Methods, 6, 351-353, 2009

Yamamoto T, Kumagai A, Saito K, Nagai T
Two-photon excitation behavior of thiophene-based oligomeres and a polymer.
J Nanosci Nanotechnol., 9, 670-672, 2009


蛋白質 核酸 酵素, 54, 1913-1917, 2009

蛋白質 核酸 酵素, 54, 1945-1951, 2009

生物物理, 49, 181-186, 2009

日本組織細胞化学会編, 組織細胞化学2009, 99-105, 2009

化学, 64, 72-73, 2009

細胞工学, 28, 402-407, 2009

分光研究, 58, 7-9, 2009

蛋白質 核酸 酵素, 54, 86-92, 2009


Saito K, Kobayashi K, Tani T, Nagai T
A mercury arc lamp-based multi-color confocal real time imaging system for cellular structure and function.
Cell Struct Funct. 33, 133-141, 2008

Kotera I, Nagai T
A high-throughput and single-tube recombination of crude PCR products using a DNA polymerase inhibitor and type IIS restriction enzyme.
J. Biotechnol. 137, 1-7, 2008

Sunabori T, Tokunaga A, Nagai T, Sawamoto K, Okabe M, Miyawaki A, Matsuzaki Y, Miyata T, Okano H.
Cell-cycle-specific nestin expression coordinates with morphological changes in embryonic cortical neural progenitors.
J Cell Sci. 121, 1204-1212, 2008

Matsuda T, Miyawaki A, Nagai T.
Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein.
Nature Methods
, 5, 339-345, 2008


実験医学, 26, 156-162, 2008

蛋白質 核酸 酵素, 53, 1858-1864, 2008

バイオインダストリー, 25, 21-26, 2008


Takemoto K, Kuranaga E, Tonoki A, Nagai T, Miyawaki A, Miura M.
Local initiation of caspase activation in Drosophila salivary gland programmed cell death in vivo..
Proc Natl Acad Sci U S A., 104, 13367-13372, 2007


Wei C, Nagai T, Wei W, Nemoto T, Awais M, Niwa O, Kurita R, Baba Y.
New advances in nanomedicine: diagnosis and preventive medicine.
Med Clin North Am., 91, 871-879, 2007




実習6 FRET、268-277

実習9 全反射顕微鏡、291-297

講義と実習 生細胞蛍光イメージング 原口徳子、木村宏、平岡泰 編集(分担執筆)、共立出版、2007




実験がうまくいく 蛍光・発光試薬の選び方と使い方 三輪佳宏 編集(分担執筆)、羊土社、2007

病理と臨床, 25, 514-519, 2007

顕微鏡, 161-165, 2007

バイオとナノの融合 I 新生命科学の基礎, 北海道大学COE研究成果編集委員会(分担執筆), 第8章, 97-113, 北海道大学、2007


Kazuno AA, Munakata K, Nagai T, Shimozono S, Tanaka M, Yoneda M, Kato N, Miyawaki A, Kato T.
Identification of mitochondrial DNA polymorphisms that alter mitochondrial matrix pH and intracellular calcium dynamics.
PLoS Genet. 2 , e128, 2006

Han YW, Tani T, Hayashi M, Hishida T, Iwasaki H, Shinagawa H, Harada Y.
Direct observation of DNA rotation during branch migration of Holliday junction DNA by Escherichia coli RuvA-RuvB protein complex.
Proc Natl Acad Sci U S A. 103, 11544-8, 2006


永井健治 (2006)
蛋白質 核酸 酵素, 51, 1989-1997

永井健治 (2006)
電顕に期待するもの 4
細胞工学, 25, 1192-1193

永井健治 (2006)
バイオテクノロジージャーナル, 6

永井健治 (2006)
細胞工学, 25, 1010-1013

永井健治 (2006)
生化学, 78, 759-763

永井健治 (2006)
Bionics, 3, 30-35

永井健治 (2006)
実験医学別冊:染色・バイオイメージング実験ハンドブック (分担執筆)羊土社, 210-215


Kohyama J, Tokunaga A, Fujita Y, Miyoshi H, Nagai T, Miyawaki A, Nakao K, Matsuzaki Y, Okano H.
Visualization of spatiotemporal activation of Notch signaling: Live monitoring and significance in neural development.
Dev. Biol., 286, 311-325, 2005

Takao K, Okamoto K, Nakagawa T, Neve RL, Nagai T, Miyawaki A, Hashikawa T, Kobayashi S, Hayashi Y.
Visualization of synaptic Ca2+ /calmodulin-dependent protein kinase II activity in living neurons.
J. Neurosci.
, 25, 3107-3112, 2005

Lin X, Varnai P, Csordas G, Balla A, Nagai T, Miyawaki A, Balla T, Hajnoczky G
Control of calcium signal propagation to the mitochondria by inositol 1,4,5-trisphosphate-binding proteins.
J. Biol Chem.
, 280, 12820-12832, 2005

Ikeda M, Ikeda-Sagara M, Okada T, Clement P, Urade Y, Nagai T, Sugiyama T, Yoshioka T, Honda K, Inoue S.
Brain oxidation is an initial process in sleep induction.
Neuroscience, 130, 1029-1040, 2005

Sasuga Y, Tani T, Hayashi M, Yamakawa H, Ohara O, Harada Y.
Development of a microscopic platform for real-time monitoring of biomolecular interactions.
Genome Research., 2005

Hirano T, Shibata S, Ohnishi K, Tani T, Aizawa S-I.
N-terminal signal region of FliK is dispensable for length control of the flagellar hook.

Molecular Microbiology, 56, 346-360, 2005

Tani T, Miyamoto Y, Fujimori KE, Taguchi T, Yanagida T, Sako Y, Harada Y.
Trafficking of ligand-receptor complex on the growth cones as an essential step for the uptake of nerve growth factor at the distal end of axon: a single-molecule analysis.
The Journal of Neuroscience, 25, 2181-2191, 2005


永井健治、宮脇敦史 (2005)
遺伝子医学Mook別冊/分子生物学実験シリーズ 図・写真で観るタンパク質構造・機能解析実験実践ガイド, メディカル ドゥ, 173-182

谷知己、原田慶恵 (2005)
生物物理, 45, 320-323

Miyawaki A , Nagai T & Mizuno H (2005)
Engineering fluorescent proteins.
Adv.Biochem Eng Biotechnol, 95, 1-15   

Miyawaki A, Nagai T & Mizuno H (2005)
Genetic probes for calcium dynamics.
Yeste et al. eds. Imaging Neurons-A Laboratory Manual-, CSHL PRESS  


Okamoto K, Nagai T, Miyawaki A, Hayashi Y.
Rapid and persistent modulation of actin dynamics regulates postsynaptic reorganization underlying bi-directional plasticity.
Nature Neurosci, 7, 1104-1112, 2004

Nagai T, Yamada S, Tominaga, T, Ichikawa M & Miyawaki A.
Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins.
Proc. Natl. Acad. Sci. USA
, 101, 10554-10559, 2004

Iwano M, Shiba H, Miwa T, Che F, Takayama S, Nagai T, Miyawaki A & Isogai A.
Ca2+ dynamics in a pollen grain and a papilla cell during pollination of Arabidopsis.
Plant Physiology, 136, 3562-3571, 2004

Nagai T & Miyawaki A.
A high-throughput method for development of FRET-based indicators for proteolysis
Biochem Biophys Res Commun., 319, 72-77, 2004

Karasawa S, Araki T, Nagai T, Mizuno H & Miyawaki A.
Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer.
Biochem. J.
, 381, 307-312, 2004

Hasan M, Friedrich R, Larkum M, Petersen C, Euler T, Duebel M, Both M, Waters J, Bujard H, Griesbeck O, Tsien RY, Nagai T, Miyawaki A & Denk W .(2004)
Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control.
, 2, 763-775, 2004


永井健治、宮脇敦史 (2004)
医学のあゆみ, 医歯薬出版, 210, 157-158

永井健治、宮脇敦史 (2004)
日本組織細胞化学会編, 学際企画

谷知己、原田慶恵 (2004)
蛋白核酸酵素別冊 バイオ高性能機器・新技術利用マニュアル(分担執筆)


Takemoto K, Nagai T, Miyawaki A & Miura M.
Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects.
J. Cell Biol.
, 160, 235-243, 2003

Fujii H, Nagai T, Shirasawa H, Doi J, Nishimatsu S, Takeda H & Sakai M.
Anteroposterior patterning in Xenopus embryos: Egg fragment assay system revealed a synergy of dorsalizing and posteriorizing embryonic domains.
Dev. Biol.
, 252, 15-30, 2003

Hino J, Nishimatsu S, Nagai T, Matsuo H, Kangawa K & Nohno T.
Coordination of BMP-3b and cerberus is required for head formation of Xenopus embryos.
Dev. Biol., 260, 138-157, 2003

Rekas A, Alattia JR, Nagai T, Miyawaki A & Ikura M. (2003)
Crystal structure of Venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity.
J. Biol. Chem., 277, 50573-50578, 2003


Miyawaki A, Nagai T & Mizuno H. (2003)
Mechanisms of protein fluorophore formation and engineering.
Curr. Opin. Chem. Biol., 7, 557-562

Nagai T & Miyawaki A. (2003)
Application of green fluorescent proteins in cell biology
Cytometry Research, 13, 1-10

永井健治、宮脇敦史 (2003)
医学のあゆみ, 医歯薬出版, 205, 193-196

Miyawaki A, Mizuno H, Nagai T & Sawano A. (2003)
Development of genetically encoded fluorescent indicators for calcium.
Methods Enzymol., 360, 202-225  

永井健治 (2003)
日本組織細胞化学会編, 学際企画, 97-108


Kato T, Ishiwata M & Nagai T.
Mitochondrial calcium response in human transformed lymphoblastoid cells.
Life Sci.
, 71, 581-590, 2002

Shimozono S, Fukano T, Nagai T, Kirino Y, Mizuno H & Miyawaki A.
Confocal imaging of subcellular Ca2+ concentrations using a dual-excitation ratiometric indicator based on green fluorescent protein.
Science STKE
, 26, 125, PL4, 2002

Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K & Miyawaki A.
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
Nature Biotechnol.
, 20, 87-90, 2002

Suzuki Y, Tani T, Sutoh K, Kamimura S.
Imaging of the fluorescence spectrum of a single fluorescent molecule by prism-based spectroscopy.
FEBS Letters, 512, 235-239, 2002


永井健治、宮脇敦史 (2002)
生物物理, 244, 305-308

Tani T, Allen R. D., Tominaga T, Naitoh Y. (2002)
Periodic tension development of the membrane of the contractile vacuole complex that governs its membrane dynamics in Paramecium.
Cell Biology International, 26, 853-860, 2002

谷知己 (2002)
比較生理生化学, 19, 128-134、2002    


Robert V, Gurlini P, Tosello V, Nagai T, Miyawaki A, DiLisa F & Pozzan T.
Beat-to-beat of mitochondrial [Ca2+] in cardiac cells.
, 20, 4998-5007, 2001

Nagai T, Sawano A, Park ES & Miyawaki A.
Circularly permuted green fluorescent protein engineered to sense Ca2+
Proc. Natl. Acad. Sci. USA, 98:,3197-3202, 2001

Mochizuki N, Yamashita S, Kurokawa K, Ohba Y, Nagai T, Miyawaki A & Matsuda M.
Spatio-temporal images of growth-factor-induced activation of Ras and Rap1.
, 411, 1065-1068, 2001

Kitaguchi T, Nakata K, Nagai T, Aruga J & Mikoshiba K.
Xenopus Polycomblike 2 (XPcl2) controls anterior to posterior patterning of the neural tissue
Dev. Genes Evol., 211, 309-314, 2001

Tani, T., Allen, R. D., Naitoh Y.
Cellular membranes that undergo cyclic changes in tension: Direct measurement of force generation by an in vitro contractile vacuole of Paramecium multimicronucleatum.
Journal of Cell Science, 114, 785-795, 2001


Nagai T, Aruga J, Minowa O, Sugimoto T, Ohno Y, Noda T & Mikoshiba. K
Zic2 regulates the kinetics of neurulation.
Proc. Natl. Acad. Sci. USA, 97, 1618-1623, 2000

Kitaguchi T, Nagai T, Nakata K, Aruga J & Mikoshiba K.
Zic3 is involved in the left-right specification of the Xenopus embryo.
Development, 127, 4787-4795, 2000

Tani T, Allen R. D., Naitoh Y.
Periodic tension development in the membrane of the in vitro contractile vacuole of Paramecium multimicronucleatum: modification by bisection, fusion and suction.
The Journal of Experimental Biology
, 203, 239-251, 2000.

Nakata K, Nagai T, Aruga J, Mikoshiba K
Xenopus Zic family and its role in neural and neural crest development.
Mech. Dev.
,75, 43-51, 1998

Aruga J, Minowa O, Yaginuma H, Kuno J, Nagai T, Noda T, Mikoshiba K.
Mouse Zic1 is involved in cerebellar development.
J. Neurosci.
,18, 284-293, 1998

Nakata K*, Nagai T*, Aruga J & Mikoshiba K.
Xenopus Zic3, a primary regulator both in neural and neural crest development.
Proc. Natl. Acad. Sci. USA, 94: 11980-11985, 1997 (*: These two authors contributed equally to this study)

Nagai T, Aruga J, Takada S, Gunthe, T, Sporle R, Schughart K, Mikoshiba K.
The expression of the mouse Zic1, Zic2, and Zic3 gene suggests an essential role for Zic genes in body pattern formation..
Dev. Biol.
, 182, 299-313, 1997

Aruga J, Nagai T, Tokuyama T, Hayashizaki Y, Okazaki Y, Chapman VM, Mikoshiba K.
The mouse Zic gene family: Homologues of the Drosophila pair-rule gene odd-paired.
J. Biol.Chem.
, 271, 1043-1047,1996

Aoki S, Yoneda I, Nagai T, Ueno N,Murakami K.
Nondestructive imaging of internal structures of frog (Xenopus laevis) by shadow-projection X-ray microtomography.
Jpn. J. Appl. Phys
., 33, 556-558, 1994

Suzuki A, Nagai T, Nishimatsu S, Sugino H, Shibai H, Murakami K, Ueno N.
Autoinduction of activin genes in Xenopus embryos.
Biochem. J.
, 298, 275-280, 1994

Nishimatsu S, Iwao M, Nagai T, Oda S, Suzuki A, Asashima M, Murakami K, Ueno N.
A carboxyl-terminal truncated version of activin receptor mediates activin signals in early Xenopus development.
FEBS Lett.
, 312, 169-173, 1992


永井健治、宮脇敦史 (2000)
実験医学別冊:ポストゲノム時代の実験講座3 宮脇敦史(編)羊土社, 118-129