Professor Takeharu Nagai, Department of Biomolecular Science and Engineering, commented in the Technology Feature in this month issue of Nature Methods. He is developing several techniques of the super-resolution imaging using photoswitchable protein Kohinoor. This article is commented "Specialty probes give super-res imaging that special blink".
See more... https://www.nature.com/articles/s41592-018-0231-8
Super-resolved image of actin filaments labeled with Kohinoor the fast photoswitchable fluorescent protein
[Tiwari et al, 2005] in a HeLa cell observed by SPoD-ExPAN [Wazawa et al, 2018]
Tiwari, D.K. et al. A fast- and positively photoswitchable fluorescent protein for ultralow-laser-power RESOLFT nanoscopy. Nat. Methods 12, 515-518 (2015)
https://www.nature.com/articles/nmeth.3362
Wazawa, T. et al. Highly biocompatible super-resolution fluorescence imaging using the fast photoswitching fluorescent protein Kohinoor and SPoD-ExPAN with Lp-regularized image reconstruction. Microscopy 67, 89-98 (2018)
https://academic.oup.com/jmicro/article/67/2/89/4835695
- Takeharu Nagai
Professor
The Institute of Scientific and Industrial Research, Osaka University
Depertment of Biomolecular Science and Engineering
Team leader of Singularity biology Project supported by JSPS
Awarded "Osaka Science Prize" in 2018.