Plasmid List

Sirius

Ultramarine fluorescent protein
Tomosugi et al., Nat. Methods, 2008
Addgene ID Name of material
51957 Sirius/pcDNA3
51956 Sirius/pRSETB

msECFP

Cyan fluorescent protein
Matsuda et al., Nat. Methods, 2008
Addgene ID Name of material
51951 mSECFP/pcDNA3

Gamillus

Acid tolerant green fluorescent protein
Shinoda et al., Cell Chem. Biol., 2018
Addgene ID Name of material
124837 Gamillus/pcDNA3

Phamret

Photo convertible fluorescent protein (Cyan to Green)
Matsuda et al., Nat. Methods, 2008
Addgene ID Name of material
51953 Phamret/pcDNA3
51955 Phamret-H2B/pcDNA3
51954 Phamret-Fibrillarin/pcDNA3

PA-TNXL

Photo activatable green fluorescent Ca2+ sensor
Matsuda et al., Sci. Rep., 2013
Addgene ID Name of material
51994 PA-TNXL/pcDNA3

Kohinoor

Photoswitchable green fluorescent protein
Tiwari et al., Nat. Methods, 2015
Addgene ID Name of material
67771 Kohinoor/pcDNA3
67770 Kohinoor/pRSETB
67776 Kohinoor-Actin/pcDNA3
67772 Kohinoor-Vimentin/pcDNA3
67777 Kohinoor-Zyxin/pcDNA3
67775 Kohinoor-Paxillin/pcDNA3
67774 Kohinoor-H2B/pcDNA3
67781 Kohinoor-Fibrilarin/pcDNA3
67779 Kohinoor-Golgi/pcDNA3
67778 Kohinoor-mitochondria/pcDNA3
67773 Kohinoor-Clathrin/pcDNA3
67780 Kohinoor-Tublin/pcDNA3

SPOON

Spontaneously blinking fluorescent protein
Arai et al., ACS Chem. Biol., 2018
Addgene ID Name of material
124840 SPOON/pcDNA3

gTEMP

Ratiometric fluorescent thermo sensor
Nakano et al., PloS One, 2017
Addgene ID Name of material
89583 gTEMP_pcDNA33
109117 mito-gTEMP_pcDNA3
109118 nuc-gTEMP_pcDNA3

Pericam

Circularly permuted GFP based Ca2+ sensor
Nagai et al., PNAS, 2001
Riken ID Name of material
RDB15144 Ratiometric pericam-mt/pcDNA3
RDB15145 Ratiometric pericam-nu/pcDNA3
RDB15139 Flash pericam/pcDNA3
RDB15140 Flash pericam/pcDNA3
RDB15141 Split pericam-N/pcDNA3
RDB15142 Split pericam-C/pcDNA3

Venus

Yellow fluorescent protein
Nagai et al., Nat. Biotech., 2002
Riken ID Name of material
RDB15116 Venus/pCS2
RDB15119 NPY-Venus/pEF-BOS

Yellow-Cameleon

Circularly permuted YFP based Ca2+ sensor
Nagai et al., PNAS, 2004
Riken ID Name of material
RDB15134 YC2.60/pRSETB
RDB15135 YC3.60/pcDNA3
RDB15136 YC3.60/pRSETB
RDB15138 YC4.60/pRSETB

Yellow Cameleon-Nano

Ultrasensitive ratiometric fluorescent Ca2+ sensor (Cyan to Yellow)
Horikawa et al., Nat. Methods, 2010
Addgene ID Name of material
51961 Yellow Cameleon-Nano15/pcDNA3
51963 Yellow Cameleon-Nano30/pcDNA3
51964 Yellow Cameleon-Nano50/pcDNA3
51965 Yellow Cameleon-Nano65/pcDNA3
51966 Yellow Cameleon-Nano140/pcDNA3

SCAT3.1

FRET-based caspase-3 indicator
Nagai and Miyawaki, BBRC, 2004
Riken ID Name of material
RDB15705 SCAT3.1/pcDNA4 HMB

ATeam1

Ratiometric fluorescent ATP sensor (Cyan to Yellow)
Kotera et al., ACS Chem. Biol., 2010
Addgene ID Name of material
51958 ATeam1.03-nD/nA/pcDNA3
51960 ATeam1.03-nD/nA/pRSETB
51959 ATeam1.03-nD/nA/pENTR1A

MARIO

Ratiometric fluorescent Mg2+ sensor (Cyan to Yellow)
Maeshima et al., Crr. Biol., 2018
Addgene ID Name of material
124838 MARIO/pcDNA3

BRAC

Ratiometric Biomiluminescent Ca2+ sensor (Cyan to Yellow)
Saito et al., Plos One, 2010
Addgene ID Name of material
51967 BRAC/pcDNA3

Nano-lantern

Saito et al., Nat. Commun., 2012
Addgene ID Name of material
51970 Nano-lantern/pcDNA3
51969 Nano-lantern/pRSETB
51974 Nano-lantern-peroxisome/pcDNA3
51971 Nano-lantern-H2B/pcDNA3
51972 Nano-lantern-Actin/pEGFP-C1
51975 Nano-lantern-plasma membrane/pcDNA3
51973 Nano-lantern-mitochondria/pcDNA3
67660 Cyan Nano-lantern/pcDNA3
67658 Cyan Nano-lantern/pRSETB
67661 Orange Nano-lantern/pcDNA3
71349 Orange Nano-lantern-H2B/pcDNA3

Biomiluminescent Ca2+ sensor based on Nano-lantern

Saito et al., Nat. Commun., 2012
Addgene ID Name of material
51987 Nano-lantern(Ca2+)_11/pcDNA3
51981 Nano-lantern(Ca2+)_11/pRSETB
51986 Nano-lantern(Ca2+)_63/pcDNA3
51980 Nano-lantern(Ca2+)_63/pRSETB
51985 Nano-lantern(Ca2+)_117/pcDNA3
51979 Nano-lantern(Ca2+)_117/pRSETB
51984 Nano-lantern(Ca2+)_150/pcDNA3
51978 Nano-lantern(Ca2+)_150/pRSETB
51983 Nano-lantern(Ca2+)_300/pcDNA3
51977 Nano-lantern(Ca2+)_300/pRSETB
51982 Nano-lantern(Ca2+)_600/pcDNA3
51976 Nano-lantern(Ca2+)_600/pRSETB
71334 Cyan Nano-lantern(Ca2+)/pcDNA3
71345 Mito-Cyan Nano-lantern(Ca2+)/pcDNA3
71346 Orange Nano-lantern(Ca2+)/pcDNA3
67659 Orange Nano-lantern(Ca2+)/pRSETB
71349 Nano-lantern(Ca2+)_11/pcDNA3

SuperNova

ROS releasing protein
Takemoto et al., Sci. Rep., 2013
Riani et al., BMC Biol., 2018
Addgene ID Name of material
53234 SuperNova/pRSETB
Addgene ID Name of material
124839 SuperNova Green/pRSETB

PACR

Caged Ca2+ releasing protein
Fukuda et al., ACS Chem. Biol., 2004
Addgene ID Name of material
55774 msEGFP-PACR/pcDNA3
55775 msEGFP-PACR/pRSETB