Publication

[ Original papers ]

Zhai L, Nakashima R, Shinoda H, Ike Y, Matsuda T and Nagai T
Structure-based analysis and evolution of a monomerized red-colored chromoprotein from the Olindias formosa jellyfish
Protein Science, 31, 2022.05

[ Books ]

永井 健治, 松田 知己, 鈴木 和志, 篠田 肇
タンパク質プローブ
先端の分析法 (監修:澤田 嗣郎), NTS, 317-323, 2022.01.29

服部 満,永井 健治
生物発光顕微鏡
先端の分析法 (監修:澤田 嗣郎), NTS, 397-400, 2022.01.29

Horikawa K and Nagai T
Live imaging of cAMP signaling in D. discoideum based on a bright bioluminescent protein, Nano-lantern (cAMP)
Methods Molecular Biology, in press, 2022

Hattori M, Itoh Y, and Nagai T
Method for measuring bioactive molecules in blood by a smartphone using bioluminescent ratiometric indicators
Bioluminescence 4th Ed, accepted. Springer Nature, 2022

[ Reviews ]

野間 涼平, 和沢 鉄一, 永井 健治
細胞に優しい超解像イメージング
CSJカレントレビュー, 印刷中, 2022

永井 健治, 市村 垂生
100万以上の細胞をミクロンレベルの空間分解能でワンショット観察可能なトランススケールスコープAMATERAS-外れ値科学の創出をめざして-
シン・マクロファージ(編集:佐藤壮), 羊土社, 実験医学, 40, 186-192, 2022

市村 垂生, 永井 健治
100万以上の細胞を同時観察可能なトランススケールスコープ
日本工業出版,画像ラボ, 33, 2022.04.10

松田 知己, 服部 満, 永井 健治
蛍光・生物発光タンパク質を利用した機能分子の開発
材料の科学と工学, 日本材料科学会, 59, 2022.04.20

[ Original papers ]

Goto A, Bota A, Miya K, Wang J, Tsukamoto S, Jiang X, Hirai D, Murayama M, Matsuda T, McHugh TJ, Nagai T and Hayashi Y
Stepwise synaptic plasticity events drive the early phase of memory consolidation
Science, 374, 857, 2021 [Press release]

Kanadome T, Hoshino N, Nagai T, Matsuda T, Yagi T
Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin
Scientific Reports, 11, 22237, 2021

Hattori M, Sugiura N, Wazawa T, Matsuda T and Nagai T
Ratiometric bioluminescent indicator for a simple and rapid measurement of thrombin activity using a smartphone
Analytical Chemistry, 93, 13520, 2021

Kubo T, Temma K, Sugiura K, Shinoda H, Lu K, Smith NI, Matsuda T, Nagai T and Fujita K
Visible-wavelength multiphoton activation confocal microscopy
ACS Photonics, 8, 2666-2673, 2021

Ichimura T, Kakizuka T, Horikawa K, Seiriki K, Kasai A, Hashimoto H, Fujita K, Watanabe TM and Nagai T
Exploring rare cellular activity in more than one million cells by a transscale scope
Scientific Reports, 11, 16539, 2021

Vu C.Q, Fukushima S, Wazawa T and Nagai T
A highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like polypeptide
Scientific Reports, 11, 16519, 2021

Kaku T, Sugiura K, Entani T, Osabe K and Nagai T
Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer
Scientific Reports, 11, 14994, 2021

Tran Q, Osabe K, Entani T and Nagai T
A novel petal up-regulated PhXTH7 promoter analysis in Petunia hybrida by using bioluminescence reporter gene
Plant Biotechnology, 38, 197-204, 2021

Wazawa T, Noma R, Uto S, Sugiura K, Washio T and Nagai T
A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging
Microscopy, 70, 340-352, 2021

Itoh Y*, Hattori M*, Wazawa T, Arai Y and Nagai T (*co-first authors)
Ratiometric bioluminescent indicator for simple and rapid diagnosis of bilirubin
ACS Sensors, 6, 889-895, 2021

Kubo T, Temma K, Smith NI, Lu K, Matsuda T, Nagai T and Fujita K
Hyperspectral two-photon excitation microscopy using visible wavelength
Optics Letters, 46, 37-40, 2021

[ Preprint ]

Kanadome T, Hoshino N, Nagai T, Matsuda T, Yagi T
Development of FRET-based indicators For visualizing homophilic trans interaction of a clustered protocadherin
Research Square, 2021

[ Books ]

Suzuki K, Hossain M.N, Matsuda T and Nagai T
Multicolor Bioluminescence Imaging of Subcellular Structures and Multicolor Calcium Imaging in Single Living Cells
Multiplexed Imaging, 229-237, Springer Nature, 2021

Hattori M, Matsuda T and Nagai T
Method for detecting emission spectral change of bioluminescent ratiometric indicators by a smartphone
Live Cell Imaging, ( Edited by Sung-Bae Kim) 295-304, Springer Nature, 2021

Riani Y, Matsuda T and Nagai T
Genetically Encoded Photosensitizer for Destruction of Protein or Cell Function
Optogenetics, (Edited by Yawo H, Kandori H, Koizumi A, Kageyama R), 265-279, Springer Nature, 2021

杉浦 一徳, 永井 健治
バイオイメージングのための蛍光タンパク質改変法
細胞組織化学2021 -生体分子の機能・局在を正しく捉えるための組織細胞化学の基礎と応用- 日本組織細胞化学会 編, 175-183, 2021

[ 解説記事 ]

永井 健治,長部 謙二 LEPによる持続可能社会の実現 -電気がなくても世界を照らす-
けいはんなView, vol.51, 6p, 2021.12

[ Others ]

永井 健治
2021年日本学士院賞:宮脇敦史博士の業績紹介
現代化学, 604, 75-76, 2021

[ Original papers ]

Hattori M, Shirane S, Matsuda T, Nagayama K and Nagai T
Smartphone-based portable bioluminescence imaging system enabling observation at various scales from whole mouse body to organelle
Sensors, 20, 7166, 2020

Harada T, Sada R, Osugi Y, Matsumoto S, Matsuda T, Hayashi Nishino M, Nagai T, Harada A and Kikuchi A
Palmitoylated CKAP4 regulates mitochondrial functions through an interaction with VDAC2 at ER-mitochondria contact sites
Journal of Cell Science, 133, jcs249045, 2020

Nakamura S, Shigeyama S, Minami S, Shima T, Akayama S, Matsuda T, Esposito A, Napolitano G, Kuma A, Namba-Hamano T, Nakamura J, Yamamoto K, Sasai M, Tokumura A, Miyamoto M, Oe Y, Fujita T, Terawaki S, Takahashi A, Hamasaki M, Yamamoto M, Okada Y, Komatsu M, Nagai T, Takabatake Y, Xu H, Isaka Y, Ballabio A and Yoshimori T
LC3 lipidation is essential for TFEB activation during the lysosomal damage response to kidney injury
Nature Cell Biology, 22, 1252–1263, 2020

Hossain M.N, Ishida R, Hattori M, Matsuda T and Nagai T
Bioluminescent Ratiometric Indicator for Analysis of Water Hardness in Household Water
Sensors, 20, 3164, 2020

Kuboki T, Ebata H, Matsuda T, Arai Y, Nagai T and Kidoaki S
Hierarchical development of motile polarity in durotactic cells just crossing an elasticity boundary
Cell Structure and Function, 45, 33-43, 2020

Araki S, Nakano M, Tsugane M, Sunaga F, Hattori M, Nagai T and Suzuki H.
A simple microfluidic device for live-imaging of the vertical section of epithelial cells
Analyst, 145, 667-674, 2020

[ Preprint ]

Ichimura T, Kakizuka T, Horikawa K, Seiriki K, Kasai A, Hashimoto H, Fujita K, Watanabe M.T and Nagai T
Trans-scale-scope to find rare cellular activity in sub-million cells
bioRχiv, 2020

Kakizuka T, Hara Y, Ohta Y, Mukai A, Ichiraku A, Arai Y, Ichimura T, Nagai T and Horikawa K
Cellular logics bringing the symmetry breaking in spiral nucleation revealed by trans-scale imaging
bioRχiv, 2020

[ Reviews ]

Nagai T, Chikuma S and Hanaoka K
Detection of singularity in immunity and cancer by novel imaging techniques
Biophysics and Physicobiology, 17, 98-99, 2020

Wazawa T, Washio T and Nagai T
Highly Biocompatible Super-resolution Imaging: SPoD-OnSPAN
Single Molecule Microscopy, Yamamoto, N. & Okada, Y. Eds., Neuromethods, 229-244, 2020

[ Proceedings ]

Kubo T, Temma K, Smith N. I, Kai L, Matsuda T, Nagai T and Fujita K
Hyperspectral fluorescence imaging by using visible-wavelength two-photon excitation
SPIE Proceedings 11521, 1152117, 2020

[ Reviews in Japanese ]

坂本 丞, 永井 健治, 亀井 保博
蛍光タンパク質温度プローブとその活用
細胞, 52, 14, 41-45, 2020

服部 満, 松田 知己, 永井 健治
バイオセンサ
炎症と免疫, 28, 71-74, 2020

篠田 肇
耐酸性可逆的光スイッチ緑色蛍光タンパク質
生物物理, 60, 176-177, 2020

松田知己, 永井健治
多色の光増感蛍光タンパク質を用いた複数機能の個別破壊
アグリバイオ, 4, 61-65, 2020

松田 知己, 永井 健治
光増感蛍光タンパク質を用いた多色機能破壊
細胞, 52, 47-50, 2020

永井 健治, 稲垣 成矩, 揚妻 正和
自由行動している複数マウスの脳活動を実時間計測する新たな技術-社会性行動を司さどる脳機能研究の新たなる展開について-
自動車技術, 74, 100-101, 2020

松田 知己, 永井 健治
高輝度発光タンパク質による高感度バイオイメージング
光学, 49, 26-30, 2020

[ Books ]

永井 健治
光るタンパク質が拓く未来社会
北海道大学公共政策大学院ブックレット, HPOS文理融合ルネッサンス -先端的政策課題のゆくえ- 遠藤乾・鈴木一人(編), 第6章, 95-107

[ Others ]

永井 健治
追想:第57回“お祭り”年会@宮崎
生物物理, 60, 192-193, 2020

[ Original papers ]

Shinoda H, Lu Kai, Nakashima R, Wazawa T, Noguchi K, Matsuda T and Nagai T
Acid-Tolerant Reversibly Switchable Green Fluorescent Protein for Super-resolution Imaging under Acidic Conditions
Cell Chemical Biology, 26, 1469-1479, 2019

Farhana I, Hossain M.N, Suzuki K, Matsuda T and Nagai T
Genetically Encoded Fluorescence/Bioluminescence Bimodal Indicators for Ca2+ Imaging
ACS Sensors, 4, 1825-1834, 2019

Oketani R, Suda H, Uegaki K, Kubo T, Matsuda T, Yamanaka M, Arai Y, Smith N, Nagai T and Fujita K
Visible-wavelength two-photon excitation microscopy with multifocus scanning for volumetric live-cell imaging
Journal of Biomedical Optics, 25, 014502, 2019

Inagaki S, Agetsuma M, Ohara S, Iijima T, Yokota H, Wazawa T, Arai Y and Nagai T
Imaging local brain activity of multiple freely moving mice sharing the same environment
Scientific Reports, 9, 7460, 2019

[ Reviews ]

Lu K, Vu CQ, Matsuda T and Nagai T
Fluorescent protein-based indicators for functional super-resolution imaging of biomolecular activities in living cells
Int. J. Mol. Sci, 20, 5784, 2019

[ Reviews in Japanese ]

前島 一博, 松田 知己, 永井 健治
蛍光マグネシウムイオンプローブの開発とそれを利用した新たな染色体凝縮メカニズムの解明
マグネシウム(Journal of Japanese society for Magnesium Research), 37, 13-18, 2019

鈴木 和志, 稲垣 成矩, 松田 知己, 永井 健治
細胞・個体イメージング用光学プローブの開発
化学工業, 70, 516-520, 2019

和沢 鉄一, 鷲尾 隆, 永井 健治
回転偏光照明蛍光顕微鏡と光スイッチング蛍光タンパク質を用いた超解像イメージング法:SPoD-OnSPAN
光アライアンス, 30, 8, 15-19, 2019

[ Books ]

永井 健治, 篠田 肇
細胞内酸性環境の理解に向けた,耐酸性GFPの開発と応用
細胞組織化学2019 -組織細胞化学の基礎と応用:分子を観る,細胞を知る,機能を探る- 日本組織細胞化学会 編, 199-207, 2019

永井 健治,小澤 岳昌(編)
最強のステップUPシリーズ発光イメージング実験ガイド
羊土社,2019

[ Original papers ]

Nezu A, Morita T, Nagai T and Tanimura A
Simultaneous monitoring of Ca2+ responses and salivary secretion in live animals reveals a threshold intracellular Ca2+ concentration for salivation
The Physiological Society, 104, 61-69, 2018

Arai Y, Takauchi H, Ogami Y, Fujiwara S, Nakano M, Matsuda T and Nagai T
Spontaneously blinking fluorescent protein for simple single laser super-resolution live cell imaging
ACS Chemical Biology, 13, 1938-1943, 2018

Komatsu N, Terai K, Imanishi A, Kamioka Y, Sumiyama K, Jin T, Okada Y, Nagai T and Matsuda M
A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging
Scientific Reports, 8, 8984, 2018

Sato R, Kawashima R, Mai Duy Luu T, Nakano M, Nagai T and Masuda S
Significance of PGR5-dependent cyclic electron flow for optimizing the rate of ATP synthesis and consumption in Arabidopsis chloroplasts
Photosynthesis Research, 139, 359-365, 2018

Suzuki K*, Onishi T*, Nakada C, Takei S, J. Daniels M, Nakano M, Matsuda T and Nagai T
Uninterrupted monitoring of drug effects in human-induced pluripotent stem cell-derived cardiomyocytes with bioluminescence Ca2+ microscopy
BMC Research Notes, 11, 313, 2018 (* equal contribution)

Riani Y D, Matsuda T, Takemoto K, Nagai T
Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation
BMC Biol, 16, 2018

Hara-Kuge S, Nishihara T, Matsuda T, Kitazono T, Teramoto T, Nagai T and Ishihara T
An improved inverse-type Ca2+ indicator can detect putative neuronal inhibition in Caenorhabditis elegans by increasing signal intensity upon Ca2+ decrease
PLoS ONE, 13, e0194707,2018

Hossain M.N, Suzuki K, Iwano M, Matsuda T and Nagai T
Bioluminescent low-affinity Ca2+ indicator for ER with multicolor calcium imaging in single living cells
ACS Chemical Biology, 13, 1862-1871, 2018

Ohta Y, Furuta T, Nagai T and Horikawa K
Red fluorescent cAMP indicator with increased affinity and expanded dynamic range
Sci. Rep., 8, 1866, 2018

Wazawa T, Arai Y, Kawahara Y, Takauchi H, Washio T, and Nagai T
Highly biocompatible super-resolution fluorescence imaging using the fast photoswitching fluorescent protein Kohinoor and SPoD-ExPAN with Lp-regularized image reconstruction
Microscopy, 67, 89-98, 2018

Kushida Y, Arai Y, Shimono K, and Nagai T
Biomimetic chemical sensing by fluorescence signals using a virus-like particle-based platform
ACS Sensors, 3, 87-92, 2018

Maeshima K*, Matsuda T*, Shindo Y*, Imamura H*, Tamura S, Imai R, Kawakami S, Nagashima R, Soga T, Noji H, Oka K and Nagai T
A transient rise in free Mg2+ ions released from ATP-Mg by its hydrolysis contributes to mitotic chromosome condensation
Current Biology, 28, 444-451.e6, 2018 (* equal contribution)

Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T
Acid-tolerant monomeric GFP from Olindias formosa
Cell Chemical Biology, 25, 330-338, 2018

[ Reviews ]

Shinoda H, Michael S and Nagai T
Fluorescent proteins for investigating biological events in acidic environments
International Journal of Molecular Science, 19, 1548, 2018

[ Books ]

永井 健治, 鈴木 和志, 稲垣 成矩
化学発光ライブイメージングの現状と展望
細胞組織化学2018 -組織細胞化学技法の基礎から最先端まで:形を観て,機能を識る- 日本組織細胞化学会 編, 185-193, 2018

原田 慶恵,永井 健治(編)
生きてるものは全部観る! イメージングの選び方・使い方100+
羊土社 実験医学増刊, 36, 2018

Editors: Nagai T, Togashi Y
Minorities and Small Numbers from Molecules to Organisms in Biology Toward a New Understanding of Biological Phenomena Springer
Springer Singapore, 2018

Nagai T, Riani YD
Optogenetic control of generation of reactive oxygen species for photo-inducible protein inactivation and cell ablation
Optogenetics, Royal Society of Chemistry, Accepted

Nagai T, Horikawa K, Saito K, Matsuda T
Genetically encoded Ca2+ indicators; expanded affinity range, color hue and compatibility with optogenetics
Application of Genetically Encoded Indicators to Mammalian Central Nervous System, Frontiers in Molecular Neuroscience, 38-42

[ Original papers ]

Toba S, Jin M, Yamada M, Kumamoto K, Matsumoto S, Yasunaga T, Fukunaga Y, Miyazawa A, Fujita S, Itoh K, Fushiki S, Hiroaki K, Wanibuchi H, Arai Y, Nagai T, and Hirotsune S
Alpha-synuclein facilitates to form short unconventional microtubules that have a unique function in the axonal transport
Sci. Rep., 7, 16386, 2017

Nozaki T, Imai R, Tanbo M, Nagashima R, Tamura S, Tani T, Joti Y, Tomita M, Hibino K, Kanemaki MT, Wendt KS, Okada Y, Nagai T, and Maeshima K
Dynamic organization of chromatin domains revealed by superresolution live-cell imaging
Molecular Cell, 67, 282-293, 2017

Aoyama M, *Yoshioka Y, Arai Y, Hirai H, Ishimoto R, Nagano K, Higashisaka K, Nagai T, *Tsutsumi Y
Intracellular trafficking of particles inside endosomal vesicles is regulated by particle size
Journal of Controlled Release, 260, 183-193, 2017

Seiriki K, Kasai A, Hashimoto T, Schulze W, Niu M, Yamaguchi S, Nakazawa T, Inoue K, Uezono S, Takada M, Naka Y, Igarashi H, Tanuma M, Waschek JA, Ago Y, Tanaka KF, Hayata-Takano A, Nagayasu K, Shintani N, Hashimoto R, Kunii Y, Hino M, Matsumoto J, Yabe H, Nagai T, Fujita K, Matsuda T, Takuma K, Baba A, and Hashimoto H
High-speed and scalable whole-brain imaging in rodents and primates
Neuron, 94, 1085-1100, 2017

Matsushita J*, Inagaki S*, Nishie T, Sakasai T, Tanaka J, Watanabe C, Mizutani K, Miwa Y, Matsumoto K, Takara K, Naito H, Kidoya H, Takakura N, Nagai T, Takahashi S and Ema M (*: co-first author)
Fluorescence and bioluminescence imaging of angiogenesis in Flk1-Nano-lantern transgenic mice
Sci. Rep., 7, 46597, 2017

Chang YF, Broyles CN, Brook F, Davies MJ, Turtle C, Nagai T and Daniels MJ
Non-invasive phenotyping and drug testing in single cardiomyocytes or beta-cells by calcium imaging and optogenetics
PLoS ONE, 12, e0174181, 2017

Nakano M, Arai Y, Kotera I, Okabe K, Kamei Y and Nagai T
Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response
PLoS ONE, 12, e0172344, 2017

Suzuki M, Sato M, Koyama H, Hara Y, Hayashi K, Yasue N, Imamura H, Fujimori T, Nagai T, E. Campbell R, and Ueno N
Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure
Development, 144, 1307-1316, 2017

Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G,J. Daniels M, Okamura Y, Matsuda T, and Nagai T
Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging
Scientific Reports, 7, 42398, 2017

Takemoto K, Iwanari H, Tada H, Suyama K, Sano A, Nagai T, Hamakubo T, and Takahashi T
Optical inactivation of synaptic AMPA receptors erases fear memory
Nature Biotechnology, 35, 38-47, 2017

Kim S, Arai Y, Tani T, Takatsuka H, Saito Y, Kawashima T, Kawakami S, Miyawaki A, and Nagai T
Simultaneous imaging of multiple cellular events using high-accuracy fluorescence polarization microscopy
Microscopy, 66, 110-119, 2017

[ Proceedings ]

和沢鉄一,新井由之,河原吉伸,中野雅裕,鷲尾隆,永井健治
遺伝子工学的に開発した蛍光プローブによる細胞生理機能超解像イメージング
The 31st Annual Conference of the Japanese Society for Artificial Intelligence, 2017

Inagaki S, and Nagai T
Bioluminescent indicator applicable to membrane voltage recording in various excitable cell types
SPIE Proceedings10251, 1025102, 2017

[ Reviews ]

Suzuki K and Nagai T
Recent progress in expanding the chemiluminescent toolbox for bioimaging
Current Opinion in Biotechnology, 48, 135-141, 2017

Nakano M, Nagai T
Thermometers for monitoring cellular temperature
Journal of Photochemistry and Photobiology, C: Photochemistry Reviews, 30, 2-9, 2017

Agetsuma M, Matsuda T, and Nagai T
Methods for monitoring signaling molecules in cellular compartments
Cell Calcium, 64, 12-19, 2017

[ Books ]

永井健治, 冨樫祐一(編)
少数性生物学
日本評論社, 2017

[ Reviews in Japanese ]

永井健治
1個と無限個の狭間に潜む新パラダイム
実験医学, 35, 3184-3189, 2017

堀川一樹,太田裕作,向井あすか,新井由之,永井健治
細胞集団シグナル伝達の少数制御
実験医学, 35, 3204-3210, 2017

鈴木和志,永井健治
高光度化学発光タンパク質の開発と生命科学研究への応用
生物物理, 57, 262-264, 2017

中野雅裕,永井健治
高輝度化学発光タンパク質の開発
生体の科学, 68(5), 462-463, 2017

和沢鉄一,新井由之,永井健治
SPoD-ExPAN超解像イメージング
顕微鏡, 77-81, 2017

[ Preprint server ]

Inagaki S, Agetsuma M, Ohara S, Iijima T, Wazawa T, Arai Y, Nagai T
In vivo brain activity imaging of interactively locomoting mice
bioRxiv, Posted October 15, 2017

Arai Y, Takauchi H, Ogami Y, Fujiwara S, Nakano M, Nagai T
A spontaneously blinking fluorescent protein for simple single laser super-resolution live cell imaging
bioRxiv, Posted October 8, 2017

[ Original papers ]

Shimada N, Saito M, Shukuri S, Kuroyanagi S, Kuboki T, Kidoaki S, Nagai T, and Maruyama A
Reversible monolayer/spheroid cell culture switching by UCST-type thermoresponsive ureido polymers
ACS Applied Materials & Interfaces, 8, 31524 - 31529, 2016

Suzuki K, Kimura T, Shinoda H, Bai G, Daniels M, Arai Y, Nakano M, and Nagai T
Five color variants of bright luminescent protein for real-time multicolor bioimaging
Nature Communications, 7, 13718, 2016
読売新聞、日刊工業新聞、NHKニュースなどで報道(報道数186件)

Lu-Walther H, Hou W, Kielhorn M, Arai Y, Nagai T, Kessels M, Qualmann B, and Heintzmann R
Nonlinear structured illumination using a fluorescent protein activating at the readout wavelength
PLoS ONE, 11, e0165148, 2016

Mochida S, Rata Scott, Hino H, Nagai T,and Novak B
Two bistable swithes govern M phase entry
Current Biology, 26, 3361-3367, 2016

Sakamaki K, Ishii MT, Sakata T, Takemoto K, Takagi C, Takeuchi A, Morishita R, Takahashi H, Nozawa A, Shinoda H, Chiba K, Sugimoto H, Saito A, Tamate S, Satou Y, Sang-Kee J, Matsuoka S, Koyamada K, Sawasaki T, Nagai T,and Ueno N
Dysregulation of a potassium channel, THIK-1, targeted by caspase-8 accelerates cell shrinkage
Biochim. Biophys. Acta Mol. Cell Res., 1863, 2766-2783, 2016

Ohta Y, Kamagata T, Mukai A, Takada S, Nagai T,and Horikawa K
Non-trivial effect of the color-exchange of a Donor/Acceptor pair in the engineering of Forster resonance energy transfer (FRET)-based indicators
ACS Chemical Biology, 11, 1816-1822, 2016

Pandey K, Ferreira PE, Ishikawa T, Nagai T, Kaneko O, and Yahata K
Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor
Scientific Reports 6, 23454, 2016

Lissek T, Obenhaus HA , Ditzel DAW, Nagai T, Miyawaki A , Sprengel R, and Hasan MT
General anesthetic conditions induce network synchrony and disrupt sensory processing in the cortex
Frontiers in Cellular Neuroscience, 4, 16, 2016

Morikawa TJ, Fujita H, Kitamura A, Horio T, Yamamoto J, Kinjo M, Sasaki A, Machiyama H, Yoshizawa K, Ichimura T, Imada K, Nagai T, and Watanabe TM
Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it
Scientific Reports, 6, 22342, 2016

[ Reviews ]

Inagaki S, and Nagai T
Current progress in genetically encoded voltage indicators for neural activity recording
Current Opinion In Chemical Biology, 33, 95-100, 2016

[ Reviews in Japanese ]

稲垣成矩,永井健治
遺伝子にコードされた膜電位センサーによる神経活動計測の現状と展望
Drug Delivery System, 31-2, 119-126, 2016

[ Original papers ]

Wazawa T, Morimoto, Nagai T. and Suzuki M
Rotational motion of rhodamine 6G tethered to actin through oligo(ethylene glycol) linkers studied by frequency-domain fluorescence anisotropy
Biophysics and Physicobiology, 12, 87-102, 2017

Hanasaki I, Uehara S, Arai Y, Nagai T and Kawano S
Threshold-free evaluation of near-surface diffusion and adsorption-dominated motion from single-molecule tracking data of single-stranded DNA through total internal reflection fluorescence microscopy
Jpn. J. Appl. Phys, 54, 125601, 2015

Iwano M, Ito K, Fujii S, Kakita M, Asano-Shimosato H, Igarashi M, Kaothien-Nakayama P, Entani T, Kanatani A, Takahisa M, Tanaka M, Komatsu K, Shiba H, Nagai T, Miyawaki A, Isogai A and Takayama S
Calcium signalling mediates self-incompatibility response in the Brassicaceae
Nature Plants, 1, 15128, 2015

Kim K, Lakhanpal G, Lu HE, Khan M, Suzuki A, Kato-Hayashi M, Narayanan R, Luyben TT, Matsuda T, Nagai T, Blanpied TA, Hayashi Y, and Okamoto K
A Temporary Gating of Actin Remodeling during Synaptic Plasticity Consists of the Interplay between the Kinase and Structural Functions of CaMKII
Neuron, 87, 813-826, 2015

Vadim PK, Matsuda T, Nagai T
MagIC, a genetically encoded fluorescent indicator for monitoring cellular Mg2+ using a non-FRET ratiometric imaging approach
Journal of Biomedical Optics, 20, 101203, 2015

Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, Fujita K and Nagai T
A fast- and positively photoswitchable fluorescent protein for ultralow-laser-power RESOLFT nanoscopy
Nature Methods, 12, 515-518, 2015 (日刊工業新聞, 産経WEST掲載)

Nezu A, Morita T,Tojyo Y, Nagai T and Tanimura A
Partial agonistic effects of pilocarpine on Ca2+ responses and salivary secretion in the submandibular glands of live animals
Experimental Physiology, 100, 640-651, 2015

Arai Y, Yamamoto T, Minamikawa T, Takamatsu T and Nagai T
Spectral fingerprinting of individual cells visualized by cavity-reflection-enhanced light-absorption microscopy
PLoS ONE, 10, e0125733, 2015

Yamanaka M, Saito K, Smith IN, Arai Y, Uegaki K, Yonemaru Y, Mochizuki K, Kawata S, Nagai T and Fujita K
Visible-wavelength two-photon excitation microscopy for fluorescent protein imaging
Journal of Biomedical Optics, 20, 101202, 2015

Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin T, Okada Y and Nagai T
Expanded palette of Nano-lantern for real-time muliti-color luminescence imaging
Proc. Natl. Acad. Sci. USA, 112, 4352-4356, 2015 (NHK 朝の関西ニュースにて放映、産経新聞・時事通信社・日刊工業新聞・日本経済新聞・毎日新聞に掲載)

Ichijo H, Hamada M, Takahashi S, Kobayashi M, Nagai T, Toyama T, Kawaguchi M
Lateralization, maturation, and anteroposterior topography in the lateral habenula revealed by ZIF268/EGR1 immunoreactivity and labeling history of neuronal activity
Neuroscience Reserach, 95, 27-37, 2015

Sugiura K, Nagai T, Nakano M, Ichinose H, Nakabayashi T, Ohta N, Hisabori T
Redox sensor proteins for highly sensitive direct imaging of intracellular redox state
Biochemical and Biophysical Research Communications, 457, 242-248, 2015

[ Reviews ]

Uno S, Tiwari DK, Kamiya M, Arai Y, Nagai T, Urano Y
A guide to use photocontrollable fluorescent proteins and synthetic smart fluorophores for nanoscopy
Microscopy, 64, 4, 263-277, 2015

Saito K, Nagai T
Recent progress in luminescent proteins development.
Current Opinion In Chemical Biology, 27, 46-51, 2015

Nagai T
Real time imaging of biological phenomena with super-duper luminescent proteins
CYTOLOGIA, 80, 1-2, 2015

[ Reviews in Japanese ]

中野雅裕,永井健治
肉眼でも観察できる!3色(水色, 黄緑色, 橙色)の高光度発光タンパク質
O plus E, 428, 505-506, 2015

[ Original papers ]

Oshima Y, Imamura T, Shintani A, Kajiura-Kobayashi H, Hibi T, Nagai T, Nonaka S, Nemoto T
Ultrasensitive imaging of Ca2+ dynamics in pancreatic acinar cells of Yellow Cameleon-Nano transgenic mice
International Journal of Molecular Sciences, 15, 19971-19986, 2014

Yoshiyama S, Chen Z, Okagaki T, Kohama K, Nasu-Kawaharada R, Izumi T, Ohshima N, Nagai T, Nakamura A
Nicotine exposure alters human vascular smooth muscle cell phenotype from a contractile to a synthetic type
Atherosclerosis, 237, 464-470, 2014

Jin M, Yamada M, Arai Y, Nagai T, Hirotsune S
Arl3 and LC8 regulate dissociation of dynactin from dynein
Nature Communications, 24, 5295, 2014

Kanemaru K, Sekiya H, Xu M, Satoh K, Kitajima N, Yoshida K, Okubo Y, Sasaki T, Moritoh S, Hasuwa H, Mimura M, Horikawa K, Matsui K, Nagai T, Iino M, Tanaka KF
In vivo visualization of subtle, transient and local activity of astrocytes using an ultrasensitive Ca2+ indicator
Cell Reports, 8, 311-318, 2014

Uehara S, Hanasaki I, Arai Y, Nagai T, Kawano S
Statistical characterisation of single-stranded DNA motion near glass surface beyond diffusion coefficient
Micro and Nano Letters, 9, 257-260, 2014

Fukuda N, Matsuda T, Nagai T
Optical control of the Ca2+ concentration in a live specimen with a genetically encoded Ca2+-releasing molecular tool
ACS Chemical biology, 9, 1197-1203, 2014

[ Reviews ]

Nagai T, Horikawa K, Saito K, Matsuda T
Genetically encoded Ca2+ indicators; expanded affinity range, color hue and compatibility with optogenetics
Frontiers in Neuroscience, 7, 25, 2014

Arai Y, Nagai T
Real-Time chemiluminescence imaging using Nano-Lantern probes
Current Protocols in Chemical Biology, 6, 221-236

Matsuda T, Nagai T
Quantitative measurement of intracellular protein dynamics using photobleaching or photoactivation of fluorescent proteins
Microscopy, 63, 403-408, 2014

[ Reviews in Japanese ]

永井健治
回折限界を超えた超解像蛍光顕微鏡
化学, 69, 21-26, 2014

松田知己,永井健治
光スイッチング機能プローブで挑む細胞の個性
生体の科学, 65, 101-106, 2014

齋藤健太,永井健治
高輝度化学発光タンパク質Nano-lanternの開発
化学と生物, 52, 646-650, 2014

永井健治
今のめり込み術のマルチ効能
生物物理, 54, 75, 2014

永井健治,松田知己
蛍光タンパク質が拓く超解像技術
化学フロンティア, 1分子ナノバイオ計測 15章

新井由之,永井健治
CCDカメラのデッドタイムを利用した光刺激法が可能にする蛍光・化学発光イメージングとオプトジェネティクスの高時間分解併用
生化学, 86, 2014

[ Original papers ]

Takemoto K, Matsuda T, Sakai N, Fu D, Noda M, Uchiyama S, Kotera I, Arai Y, Horiuchi M, Fukui K, Ayabe T, Inagaki F, Suzuki H, Nagai T
SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
Scientific Reports, 3, 2629, 2013

Nozaki T, Kaizu K, Pack C, Tamura S, Tani T, Hihara S, Nagai T, Takahashi K, Maeshima K
Flexible and dynamic nucleosome fiber in living mammalian cells
Nucleus, 4, 1-8, 2013

Yamanaka M, Saito K, Smith NI, Kawata S, Nagai T, Fujita K
Saturated excitation (SAX) of fluorescent proteins for sub-diffraction-limited imaging of living cells in three dimensions
Interface Focus, 3, 20130007, 2013

Yamada M, Kumamoto K, Mikuni S, Arai Y, Kinjo M, Nagai T, Tsukasaki Y, Watanabe TM, Fukui M, Jin M, Toba S, Hirotsune S
Rab6a releases LIS1 from a dynein idling complex and activates dynein for retrograde movement
Nature Communications, 4, 2033, 2013

Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang Y-F, Araki S, Arai Y, March K, Thomas H, Sagou K, Miyata T, Nagai T, Li W, Campbell R
Improved orange and red Ca2+ indicators and photophysical considerations for optogenetic applications
ACS Chemical Neuroscience, 4, 963-972, 2013

Matsuda T, Horikawa K, Saito K, Nagai T
Highlighted Ca2+ imaging with a genetically encoded 'caged' indicator
Scientific Reports, 3, 1398, 2013

Saito K, Higuchi Y, Arai Y, and Nagai T
Video-rate imaging of luminescent tumour cells in freely moving unshaved mice
Protocol Exchange

Hoi H, Matsuda T, Nagai T, Campbell RE
Highlightable Ca2+ indicators for live cell imaging
JACS, 135, 46-49, 2013

[ Reviews ]

Arai.Y, and Nagai T
Extensive use of FRET in biological imaging
Microscopy, 62, 419–428, 2013

Tiwari D.K., and Nagai T
Smart fluorescent proteins: Innovation for barrier-free superresolution imaging in living cells
Development Growth and Differentiation, 55, 491-507, 2013

Koldenkova V.P. and Nagai T
Genetically encoded Ca2+ indicators: properties and evaluation
BBA - Molecular Cell Research, 1833, 1787-1797, 2013

[ Reviews in Japanese ]

永井健治,齋藤健太,初谷紀幸
化学発光タンパク質の高輝度化とバイオイメージングへの展開
顕微鏡, 48, 1-3, 2013

堀川一樹,永井健治
Ca2+イメージングで解き明かすCa2+動態の時空間統御機構
CLINICAL CALCIUM -循環器疾患とカルシウム・リン, 23, 2013

ビジュアルバイオロジー-タンパク質でできた蛍光Ca2+センサーの開発から-
生産と技術, 65, 2, 2013

[ Original papers ]

Hihara S, Pack CG, Hanafusa T, Kaizu K, Tani T, Takemoto S, Yoshimi T, Sako Y, Yokota H, Imamoto N, Kinjo M, Takahashi K, Nagai T, Maeshima K
Local nucleosome dynamics facilitate chromatin accessibility in living mammalian cells
Cell Reports, 2, 1645-56, 2012

Saito K, Chang Yu-Fen, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, Yoshida Y, Matsuda T, Arai Y, Nagai T
Luminescent protein for high-speed single-cell and whole-body imaging
Nature Communications, 3, 1262, 2012
Drug Discovery & Development誌にて当研究成果が紹介。Nature Methods,Nature Photonicsで紹介。Faculty of 1000にノミネート。

Kominami K, Nagai T, Sawasaki T, Tsujimura Y,Yashima K, Sunaga Y, Tsuchimochi M, Nishimura J, Chiba K, Nakabayashi J, Koyamada K, Endo Y, Yokota H, Miyawaki A, Manabe N, Sakamaki K
In vivo imaging of hierarchical spatiotemporal activation of caspase-8 during apoptosis
PLoS ONE, 7, e50218, 2012

Hatsugai N, Koldenkova VP, Imamura H, Noji H, Nagai T
Changes in cytosolic ATP levels and intracellular morphology during bacteria- induced hypersensitive cell death as revealed by real-time fluorescence microscopy imaging
Plant and Cell Physiology, 53, 1768-1775, 2012

Iwano M, Ngo QA, Entani T, Shiba H, Nagai T, Miyawaki A, Isogai A,Grossniklaus U, Takayama S
Cytoplasmic Ca2+ changes dynamically during the interaction of the pollen tube with synergid cells
Development, 139, 4202-4209, 2012

Kwon HJ, Ohmiya Y, Honma K-i, Honma S,Nagai T,Saito K, Yasuda K
Synchronized ATP oscillations have a critical role in prechondrogenic condensation during chondrogenesis
Cell Death and Disease, 3, e278, 2012

Kominami K, Nakabayashi J, Nagai T, Tsujimura Y, Chiba K, Kimura H, Miyawaki A, Sawasaki T, Yokota H, Manabe N, Sakamaki K
The molecular mechanism of apoptosis upon caspase-8 activation: Quantitative experimental validation of a mathematical model.
Biochim Biophys Acta-Molecular Cell Research, 1823, 1825-1840, 2012

Chang Y, Arai Y, Nagai T
Optogenetic activation during detector 'dead time' enables compatible real-time fluorescence imaging
Neuroscience Research, 73, 341-347, 2012

Ohtsuka K, Sato S, Sato Y, Sota K, Ohzawa S, Matsuda T, Takemoto K, Takamune N, Juskowiak B, Nagai T, Takenaka S T
Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer peptide conjugate
Chemical Communications, 48, 4740-4742, 2012

[ Reviews, Books ]

永井健治
おもろいバイオロジー "蛍光プローブ開発秘話その2~夏秋冬春~"
細胞工学, 31, 2012

[ Original papers ]

Saito K, Arai Y, Zhang J, Kobayashi K, Tani T, Nagai T
Conjugation of both on-axis and off-axis light in Nipkow disk confocal microscope to increase availability of incoherent light source
Cell Struct. Func., 36, 237-246, 2011

Yamada Y, Michikawa T, Hashimoto M, Horikawa K, Nagai T, Miyawaki A,Hausser M, Mikoshiba K
Quantitative comparison of genetically encoded Ca2+ indicators in cortical pyramidal cells and cerebellar Purkinje cells
Frontiers in Cellular Neuroscience, 5, 18, 2011

Zhao Y, Araki S, Wu J, Teramoto T, Chang Y, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
An expanded palette of genetically encoded Ca2+ indicators
Science, 333, 1888-1891, 2011

Nakano M, Imamura H, Nagai T, Noji H
Ca2+ regulation of mitochondrial ATP synthesis visualized at the single cell level
ACS Chemical Biology, 6, 709-15, 2011

Nomura M, Nagai T, Harada Y, Tani T
Facilitated intracellular transport of TrkA by an interaction with nerve growth factor
Developmental Neurobiology, 71, 634-649, 2011

Takemoto K, Matsuda T, McDougall M, Klaubert DH, Hasegawa A, Los GV, Wood KV, Miyawaki A, Nagai T
Chromophore-assisted light inactivation of HaloTag fusion proteins labeled with eosin in living cells
ACS Chemical Biology, 6, 401-406, 2011

Yang L, Matsuda T, Raviraj V, Ching YW, Braet F, Nagai T, Soon LL
Imaging the dynamics of intracellular protein translocation by photoconversion of Phamret-Cybr/ROM
Journal of Microscopy, 242, 250-261, 2011

[ Reviews, Books ]

齋藤 健太,永井 健治
生物発光を利用した細胞内カルシウムイメージング
生物物理, 52, 2011

永井 健治,堀川 一樹
機能イメージングにおける指示薬感度の重要性―蛍光タンパク質間FRETを用いたCa2+指示薬開発からの考察―
蛍光イメージング/MRIプローブの開発
CMC出版

堀川 一樹,永井 健治
細胞性粘菌の集合流形成における細胞間シグナル伝達
生物の科学, 遺伝 65, 87-91, 2011

斎藤 健太,永井 健治
化学発光を利用した生命機能の可視化
化学と生物, 49, 555-559, 2011

[ Original papers ]

Horikawa K, Yamada Y, Matsuda T, Kobayashi K, Hashimoto M, Matsu-ura T, Miyawaki A, Michikawa T, Mikoshiba K, Nagai T
Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano
Nature Methods, 7, 729-732, 2010

Saito K, Hatsugai N, Horikawa K, Kobayashi K, Matsu-Ura T, Mikoshiba K, Nagai T
Auto-luminescent genetically-encoded ratiometric indicator for real-time Ca2+ imaging at the single cell level
PLoS ONE, 5, e9935,2010

Lutcke H, Murayama M, Hahn T, Margolis DJ, Astori S, Borgloh SMzA, Gobel W, Yang Y, Tang W, Kugler S, Sprengel R, Nagai T, Miyawaki A, Larkum ME, Helmchen F, Hasan MT
Optical recording of neuronal activity with a genetically-encoded Ca2+ indicator in anesthetized and freely moving mice
Frontiers in Neural Circuit, 4, 1-12, 2010

Hong JH, Min CH, Jeong B, Kojiya T, Morioka E, Nagai T, Ikeda M. Lee K
Intracellular calcium spikes in rat suprachiasmatic nucleus neurons induced by BAPTA-based calcium dyes
PLoS ONE, 5, e9634,2010

Kotera I, Iwasaki T, Imamura H, Noji H, Nagai T
Reversible dimerization of Aequorea victoria fluorescent proteins increases the dynamic range of FRET-based indicators
ACS Chemical Biology, 5, 215-222, 2010

[ Reviews, Books ]

永井健治
なぜなぜ生物学
細胞の中って見えるの?
日本分子生物学会, 第十二章 153-167, 2010

永井健治
1枚の写真館 幼名と改名
細胞工学, 29, 843, 2010

永井健治
Dual FRET法を利用した複数の生理機能イメージング
シングルセル解析の最前線, 神原秀記, 松永是, 植田充美 監修 (分担執筆)
第一章 61-69, シーエムシー出版, 2010

永井健治
蛍光タンパク質エンジニアリングによるバイオイメージング技術の進展
レーザー研究, 38, 416-420, 2010

永井健治,小林健太郎
1波長励起Dual FRETによる生細胞内における複数現象の可視化
実験医学, 28, 589-598, 2010

[ Original papers ]

Iwano M, Entani T, Shiba H, Kakita H, Nagai T, Mizuno H, Miyawaki A, Shoji T, Kubo K, Isogai A, Takayama S
Fine-tuning of the cytoplasmic Ca2+ concentration is essential for pollen tube growth
Plant Physiology, 150, 1322-1334, 2009

Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa K, Nagai T
An ultramarine fluorescent protein with increased photostability and pH insensitivity
Nature Methods, 6, 351-353, 2009

Yamamoto T, Kumagai A, Saito K, Nagai T
Two-photon excitation behavior of thiophene-based oligomeres and a polymer
J Nanosci Nanotechnol., 9, 670-672, 2009

Imamura H, Nhat KPH, Togawa H, Saito K, Iino R, Kato-Yamada Y, Nagai T, Noji H
Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators
Proc. Natl, Aca, Sci. U.S.A., 106, 15651-15656, 2009

[ Reviews, Books ]

野馳博行,永井健治
生命システムの階層間をまたぐイメージング技術
蛋白質 核酸 酵素, 54, 1913-1917, 2009

堀川一樹,永井健治
細胞結合振動しシステムを対象にした超高感度大規模イメージング
蛋白質 核酸 酵素, 54, 1945-1951, 2009

永井健治,松田知己
生細胞内でのタンパク質動態測定とその意義
生物物理, 49, 181-186, 2009

永井健治,松田知己
光スイッチング蛍光タンパク質を用いた生細胞内でのタンパク質動態定量法
日本組織細胞化学会編, 組織細胞化学2009, 99-105, 2009

永井健治
酸性の環境でも光る蛍光タンパク質
化学, 64, 72-73, 2009

小寺一平,永井健治
ワンステップ全自動プラスミド構築方法
細胞工学, 28, 402-407, 2009

永井健治
光スイッチング蛍光タンパク質を用いた超解像観察法
分光研究, 58, 7-9, 2009

永井健治
蛍光プロープ開発秘話:Pericam
蛋白質 核酸 酵素, 54, 86-92, 2009

[ Original papers ]

Saito K, Kobayashi K, Tani T, Nagai T
A mercury arc lamp-based multi-color confocal real time imaging system for cellular structure and function
Cell Struct Funct. 33, 133-141, 2008

Kotera I, Nagai T
A high-throughput and single-tube recombination of crude PCR products using a DNA polymerase inhibitor and type IIS restriction enzyme
J. Biotechnol. 137, 1-7, 2008

Sunabori T, Tokunaga A, Nagai T, Sawamoto K, Okabe M, Miyawaki A, Matsuzaki Y, Miyata T, Okano H
Cell-cycle-specific nestin expression coordinates with morphological changes in embryonic cortical neural progenitors
J Cell Sci. 121, 1204-1212, 2008

Matsuda T, Miyawaki A, Nagai T
Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein
Nature Methods, 5, 339-345, 2008

[ Reviews, Books ]

永井健治,松田知己
光変換蛍光タンパク質を用いた生体分子の動態解析法
実験医学, 26, 156-162, 2008

松田知己,永井健治
新規の光変換蛍光蛋白質プローブを用いた生体イメージングと動態解析
蛋白質 核酸 酵素, 53, 1858-1864, 2008

松田知己,永井健治
光活性化・光変換蛍光タンパク質を用いたタンパク質動態解析
バイオインダストリー, 25, 21-26, 2008

[ Original papers ]

Takemoto K, Kuranaga E, Tonoki A, Nagai T, Miyawaki A, Miura M
Local initiation of caspase activation in Drosophila salivary gland programmed cell death in vivo
Proc Natl Acad Sci U S A., 104, 13367-13372, 2007

[ Reviews, Books ]

Wei C, Nagai T, Wei W, Nemoto T, Awais M, Niwa O, Kurita R, Baba Y
New advances in nanomedicine: diagnosis and preventive medicine
Med Clin North Am., 91, 871-879, 2007

永井健治, 改変型蛍光タンパク質の利用, 67-78
永井健治, 小寺一平, 共鳴エネルギー移動(FRET)の基礎, 110-117
永井健治, 斉藤健太, FRETの測定法と評価, 118-128
斉藤健太, 松田知己, 原口徳子, 永井健治, 実習6 FRET, 268-277
小寺一平, 谷知己, 永井健治, 実習9 全反射顕微鏡, 291-297
講義と実習 生細胞蛍光イメージング, 共立出版, 2007

竹本研, 永井健治,宮脇敦史,三浦正幸, アポトーシスの検出,143-146
谷知己,永井健治, Column:スパイはひとりで充分,147
小寺一平,永井健治, FRETプローブ,158-162
実験がうまくいく 蛍光・発光試薬の選び方と使い方,羊土社,2007

永井健治
蛍光イメージング─最近の進歩:回折限界の壁を越える工夫─
病理と臨床, 25, 514-519, 2007

齊藤健太,谷知己,小林健太郎,永井健治
大口径ファイバー白色光源を用いた高速共焦点顕微鏡の開発と評価
顕微鏡, 161-165, 2007

永井健治
発光性蛋白質を利用したバイオセンサーの開発
バイオとナノの融合 I 新生命科学の基礎, 北海道大学COE研究成果編集委員会, 第8章, 97-113, 北海道大学,2007

[ Original papers ]

Kazuno AA, Munakata K, Nagai T, Shimozono S, Tanaka M, Yoneda M, Kato N, Miyawaki A, Kato T.
Identification of mitochondrial DNA polymorphisms that alter mitochondrial matrix pH and intracellular calcium dynamics
PLoS Genet. 2 , e128, 2006

[ Reviews, Books ]

永井健治
FRETの上手な使い方
蛋白質 核酸 酵素, 51, 1989-1997, 2006

永井健治
電顕に期待するもの 4
細胞工学, 25, 1192-1193, 2006

永井健治
HaloTagテクノロジーが拓く様々な蛍光イメージングの可能性
バイオテクノロジージャーナル, 6, 2006

永井健治
組織・個体レベルでの機能イメージングに向けて
細胞工学, 25, 1010-1013, 2006

永井健治
今、蛍光蛋白質で何ができるか?
生化学, 78, 759-763, 2006

永井健治
生細胞内ではたらく分子を可視化する
Bionics, 3, 30-35, 2006

永井健治, 小寺一平
円順列GFP変異体を利用した機能プローブの作製法と2波長励起1波長取得共焦点イメージング
実験医学別冊:染色・バイオイメージング実験ハンドブック, 羊土社, 210-215, 2006

[ Original papers ]

Kohyama J, Tokunaga A, Fujita Y, Miyoshi H, Nagai T, Miyawaki A, Nakao K, Matsuzaki Y, Okano H
Visualization of spatiotemporal activation of Notch signaling: Live monitoring and significance in neural development
Dev. Biol., 286, 311-325, 2005

Takao K, Okamoto K, Nakagawa T, Neve RL, Nagai T, Miyawaki A, Hashikawa T, Kobayashi S, Hayashi Y
Visualization of synaptic Ca2+ /calmodulin-dependent protein kinase II activity in living neurons
J. Neurosci., 25, 3107-3112, 2005

Lin X, Varnai P, Csordas G, Balla A, Nagai T, Miyawaki A, Balla T, Hajnoczky G
Control of calcium signal propagation to the mitochondria by inositol 1,4,5-trisphosphate-binding proteins
J. Biol Chem., 280, 12820-12832, 2005

Ikeda M, Ikeda-Sagara M, Okada T, Clement P, Urade Y, Nagai T, Sugiyama T, Yoshioka T, Honda K, Inoue S
Brain oxidation is an initial process in sleep induction
Neuroscience, 130, 1029-1040, 2005

[ Reviews, Books ]

永井健治,宮脇敦史
GFPを利用した蛍光バイオセンサーの作成法と生体機能の可視化
遺伝子医学Mook別冊/分子生物学実験シリーズ 図・写真で観るタンパク質構造・機能解析実験実践ガイド, メディカル ドゥ, 173-182, 2005

Miyawaki A , Nagai T & Mizuno H
Engineering fluorescent proteins
Adv.Biochem Eng Biotechnol, 95, 1-15, 2005

Miyawaki A, Nagai T & Mizuno H
Genetic probes for calcium dynamics
Yeste et al. eds. Imaging Neurons-A Laboratory Manual-, CSHL PRESS, 2005

[ 2004~2001 Original papers ]

Okamoto K, Nagai T, Miyawaki A, Hayashi Y
Rapid and persistent modulation of actin dynamics regulates postsynaptic reorganization underlying bi-directional plasticity
Nature Neurosci, 7, 1104-1112, 2004

Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A
Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins
Proc. Natl. Acad. Sci. USA, 101, 10554-10559, 2004

Iwano M, Shiba H, Miwa T, Che F, Takayama S, Nagai T, Miyawaki A, Isogai A
Ca2+ dynamics in a pollen grain and a papilla cell during pollination of arabidopsis
Plant Physiology, 136, 3562-3571, 2004

Nagai T, Miyawaki A
A high-throughput method for development of FRET-based indicators for proteolysis
Biochem Biophys Res Commun., 319, 72-77, 2004

Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A
Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer
Biochem. J., 381, 307-312, 2004

Hasan M, Friedrich R, Larkum M, Petersen C, Euler T, Duebel M, Both M, Waters J, Bujard H, Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W
Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control
PLOS, 2, 763-775, 2004

Takemoto K, Nagai T, Miyawaki A, Miura M
Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects
J. Cell Biol., 160, 235-243, 2003

Fujii H, Nagai T, Shirasawa H, Doi J, Nishimatsu S, Takeda H, Sakai M
Anteroposterior patterning in Xenopus embryos: Egg fragment assay system revealed a synergy of dorsalizing and posteriorizing embryonic domains
Dev. Biol., 252, 15-30, 2003

Hino J, Nishimatsu S, Nagai T, Matsuo H, Kangawa K, Nohno T
Coordination of BMP-3b and cerberus is required for head formation of Xenopus embryos
Dev. Biol., 260, 138-157, 2003

Rekas A, Alattia JR, Nagai T, Miyawaki A, Ikura M
Crystal structure of Venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity
J. Biol. Chem., 277, 50573-50578, 2003

Kato T, Ishiwata M, Nagai T
Mitochondrial calcium response in human transformed lymphoblastoid cells
Life Sci., 71, 581-590, 2002

Shimozono S, Fukano T, Nagai T, Kirino Y, Mizuno H, Miyawaki A
Confocal imaging of subcellular Ca2+ concentrations using a dual-excitation ratiometric indicator based on green fluorescent protein
Science STKE, 26, 125, PL4, 2002

Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
Nature Biotechnol., 20, 87-90, 2002

Robert V, Gurlini P, Tosello V, Nagai T, Miyawaki A, DiLisa F, Pozzan T
Beat-to-beat of mitochondrial [Ca2+] in cardiac cells
EMBO J., 20, 4998-5007, 2001

Mochizuki N, Yamashita S, Kurokawa K, Ohba Y, Nagai T, Miyawaki A, Matsuda M
Spatio-temporal images of growth-factor-induced activation of Ras and Rap1
Nature, 411, 1065-1068, 2001

Kitaguchi T, Nakata K, Nagai T, Aruga J & Mikoshiba K
Xenopus Polycomblike 2 (XPcl2) controls anterior to posterior patterning of the neural tissue
Dev. Genes Evol., 211, 309-314, 2001

Nagai T, Sawano A, Park ES, Miyawaki A
Circularly permuted green fluorescent protein engineered to sense Ca2+
Proc. Natl. Acad. Sci. USA, 98, 3197-3202, 2001

[ 2004~2001 Reviews, Books ]

永井健治,宮脇敦史
GFP間FRETに基づく高性能バイオセンサーの開発
医学のあゆみ, 医歯薬出版, 210, 157-158, 2004

永井健治,宮脇敦史
GFPを使ったバイオセンサーの作成法
日本組織細胞化学会編, 学際企画, 2004

Miyawaki A, Nagai T, Mizuno H
Mechanisms of protein fluorophore formation and engineering
Curr. Opin. Chem. Biol., 7, 557-562, 2003

Nagai T, Miyawaki A
Application of green fluorescent proteins in cell biology
Cytometry Research, 13, 1-10, 2003

永井健治,宮脇敦史
運動器のダイナミクスに迫るには-蛍光蛋白質を用いたバイオイメージング
医学のあゆみ, 医歯薬出版, 205, 193-196, 2003

Miyawaki A, Mizuno H, Nagai T, Sawano A
Development of genetically encoded fluorescent indicators for calcium
Methods Enzymol., 360, 202-225, 2003

永井健治
GFPを用いた方法の新たな発展
日本組織細胞化学会編, 学際企画, 97-108, 2003

永井健治,宮脇敦史
高効率に発光構造をとるGFP変異体の開発
生物物理, 244, 305-308, 2002

[ 2000~1992 Original papers ]

Nagai T, Aruga J, Minowa O, Sugimoto T, Ohno Y, Noda T, Mikoshiba K
Zic2 regulates the kinetics of neurulation
Proc. Natl. Acad. Sci. USA, 97, 1618-1623, 2000

Kitaguchi T, Nagai T, Nakata K, Aruga J, Mikoshiba K
Zic3 is involved in the left-right specification of the Xenopus embryo
Development, 127, 4787-4795, 2000

Nakata K, Nagai T, Aruga J, Mikoshiba K
Xenopus Zic family and its role in neural and neural crest development
Mech. Dev., 75, 43-51, 1998

Aruga J, Minowa O, Yaginuma H, Kuno J, Nagai T, Noda T, Mikoshiba K
Mouse Zic1 is involved in cerebellar development
J. Neurosci., 18, 284-293, 1998

Nakata K*, Nagai T*, Aruga J, Mikoshiba K
Xenopus Zic3, a primary regulator both in neural and neural crest development
Proc. Natl. Acad. Sci. USA, 94, 11980-11985, 1997 (*: These two authors contributed equally to this study)

Nagai T, Aruga J, Takada S, Gunthe, T, Sporle R, Schughart K, Mikoshiba K
The expression of the mouse Zic1, Zic2, and Zic3 gene suggests an essential role for Zic genes in body pattern formation
Dev. Biol., 182, 299-313, 1997

Aruga J, Nagai T, Tokuyama T, Hayashizaki Y, Okazaki Y, Chapman VM, Mikoshiba K
The mouse Zic gene family. Homologues of the Drosophila pair-rule gene odd-paired
J. Biol.Chem., 271, 1043-1047, 1996

Aoki S, Yoneda I, Nagai T, Ueno N, Murakami K
Nondestructive imaging of internal structures of frog (Xenopus laevis) by shadow-projection X-ray microtomography
Jpn. J. Appl. Phys., 33, 556-558, 1994

Suzuki A, Nagai T, Nishimatsu S, Sugino H, Shibai H, Murakami K, Ueno N
Autoinduction of activin genes in early Xenopus embryos
Biochem. J., 298, 275-280, 1994

Nishimatsu S, Iwao M, Nagai T, Oda S, Suzuki A, Asashima M, Murakami K, Ueno N
A carboxyl-terminal truncated version of activin receptor mediates activin signals in early Xenopus development
FEBS Lett., 312, 169-173, 1992

[ Reviews, Books ]

永井健治,宮脇敦史
アフリカツメガエル
実験医学別冊:ポストゲノム時代の実験講座3 宮脇敦史(編)羊土社, 118-129, 2000