NAGAI Laboratory[ Original papers ]
Taro Ichimura, Taishi Kakizuka, Yuki Sato, Yoichiro Fujioka, Yusuke Ohba, Kazuki Horikawa, Takeharu Nagai
Strength in numbers: Unleashing the potential of trans-scale scope AMATERAS for massive cell quantification
Biophysics and Physicobiology, e211017 2024.3
Takeharu Nagai
Editorial: Singularity Biology and Beyond
Biophysics and Physicobiology, e211013 2024.3
Tomoki Matsuda, Shinya Sakai, Kei-ichi Okazaki, Takeharu Nagai
Improvement of the Green–Red Förster Resonance Energy Transfer-Based Ca2+ Indicator by Using the Green Fluorescent Protein, Gamillus, with a Trans Chromophore as the Donor
ACS Sensors, vol 708(14):149799, 2024.3
Jiayang Wu, Yutaka Shindo, Kohji Hotta, Cong Quang Vu, Kai Lu, Tetsuichi Wazawa, Takeharu Nagai, Kotaro Oka
Calcium-induced upregulation of energy metabolism heats neurons during neural activity
Biochemical and Biophysical Research Communications, vol 708(14):149799, 2024.3
Hiroko Bannai, Akihiko Takashima, Yoshiyuki Soeda, Hideaki Yoshimura, Gen Matsumoto, Naruhiko Sahara, Michio Hiroshima, Mitsuru Hattori, Takeharu Nagai
Research on the molecular mechanism of singularity phenomenon in neurological disorders
Biophysics and Physicobiology, e211008, 2024.2
Takashi Kanadome, Natsumi Hoshino, Takeharu Nagai, Takehi Yagi, Tomoki Matsuda
Protocol to visualize trans-interaction of clustered protocadherin using cIPAD, a fluorescent indicator, in cultured human cells and mouse neurons
STAR Protocols 5(1) 102844-102844
[ Books ]
垣塚太志, 市村垂生, 永井健治
オルガノイドがもたらすライフサイエンス革命
実験医学増刊 Vol42, No.5, p.154-160, 羊土社, 2024.3
[ Reviews in Japanese ]
[ Reviews ]
[ Original papers ]
Mügen Terzioglu, Kristo Veeroja, Toni Montonen, Teemu O. Ihalainen, Tiina S. Salminen, Paule Bénit, Pierre Rustin, Young-Tae Chang, Takeharu Nagai, Howard T. Jacobs
Mitochondrial temperature homeostasis resists external metabolic stresses
eLife, 12:RP89232, 2023.11
Masakazu Agetsuma, Issei Sato, Yasuhiro R. Tanaka, Luis Carrillo-Reid, Atsushi Kasai, Atsushi Noritake, Yoshiyuki Arai, Miki Yoshitomo, Takashi Inagaki, Hiroshi Yukawa, Hitoshi Hashimoto, Junichi Nabekura & Takeharu Nagai
Activity-dependent organization of prefrontal hub-networks for associative learning and signal transformation
Nature communications, 14:5996, 2023.10
Natsumi Hoshino, Takashi Kanadome, Tomomi Takasugi, Mizuho Itoh, Ryosuke Kaneko, Yukiko U. Inoue, Takayoshi Inoue, Takahiro Hirabayashi, Masahiko Watanabe, Tomoki Matsuda, Takeharu Nagai, Etsuko Tarusawa, Takeshi Yagi
Visualization of trans homophilic interaction of clustered protocadherin in neurons
Proceedings of the National Academy of Sciences, 120(38), 2023.09
Kanadome T, Hoshino N, Nagai T, Yagi T, Matsuda T,
Visualization of trans-interactions of a protocadherin-α between processes originating from single neurons
iScience, 26, 107238, 2023.07
Kenta Temma, Ryosuke Oketani, Toshiki Kubo, Kazuki Bando, Shunsuke Maeda, Kazunori Sugiura, Tomoki Matsuda, Rainer Heintzmann, Tatsuya Kaminishi, Koki Fukuda, Maho Hamasaki, Takeharu Nagai, Katsumasa Fujita
Selective plane activation structured illumination microscopy
bioRxiv, 2023.6
Wazawa T, Nagai T
Joule heating involving ion currents through channel proteins
Biophysics and Physicobiology, 20, e200030, 2023.03
[ Books ]
Noma R，Wazawa T，Nagai T
細胞に優しい超解像イメージング
CSJ Current Review 45『生体分子環境の化学』，日本化学会編，p.137-143，化学同人, 2023.2.20
坂本 丞，Lu Kai，和沢鉄一，永井健治，亀井保博
遺伝子コード可能な温度プローブを利用した高速生体温度イメージングによる熱物性解析
『温度ストレスによる生体応答ダイナミクス』, 第8章・解析/計測技術, 第1節, NTS, 2023.05
[ Reviews in Japanese ]
市村垂生、垣塚太志、永井健治、糸賀祐弥、大浪修一
細胞画像ビックデータによるバイオDX
光アライアンス Vol. 34, No. 11『生体イメージングの最前線』，p.48-52，日本工業出版，2023
京卓志、永井健治、松田知己
蛍光タンパク質プローブによる細胞間相互作用の可視化
医学のあゆみ Vol. 286, No. 5『生体イメージングの最前線』，p.329-335，医歯薬出版，2023
和沢鉄一，魯 慨，永井健治
遺伝的にコードされた高速応答蛍光性温度指示薬によるミリ秒細胞内熱輸送プロセスの観察
実験医学 Vol. 41, No. 1『中分子ペプチド医薬で新たな標的を狙う!!』，p.91-94，羊土社，2023.01.01
和沢鉄一，魯 慨，永井健治
細胞内の温度を0.1℃の分解能，そしてサブミリ秒の分解能で測定する蛍光タンパク質の開発
月刊・細胞 Vol. 55, No. 5『蛍光タンパク質センサー』，p.284-287，ニューサイエンス社，2023.03.31
市村垂生，糸賀裕弥，垣塚太志，大浪修一，永井健治
巨大データを生み出すトランススケールスコープとその取扱いを可能にする情報プラットフォーム
光技術コンタクト Vol. 61, No. 4『大規模データセンターの構成要素技術と光学分野で脚光を浴びる活用技術』，p.43-53，日本オプトメカトロニクス協会，2023.04
田中 陸登，杉浦 一徳，服部 満，永井 健治
蛍光タンパク質・生物発光タンパク質を用いるバイオイメージング
化学と教育 Vol71, No.5『イメージングの化学』, p.192-195, 日本化学会, 2023.05
永井 健治
先導的学際研究機構・超次元ライフイメージング部門の活動
生産と技術 Vol75, No.4, p.89-93, 一般社団法人生産技術振興協会, 2023.10
[ Reviews ]
[ Original papers ]
A Jahana, MST T Aktera, K Takemoto, T Oura, A Shitara, S Semba, A Nezu, S Suto, T Nagai & A Tanimura
Insertion of circularly permuted cyan fluorescent protein into the ligand-binding domain of inositol 1,4,5-trisphosphate receptor for enhanced FRET upon binding of fluorescent ligand
Cell calcium, 108, 102668, 2022.12
Sugiura K & Nagai T
Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application
Communications Biology, 5, 1172, 2022.11
Kanadome T, Hayashi K, Seto Y, Eiraku M, Nakajima K, Nagai T & Matsuda T
Development of intensiometric indicators for visualizing N-cadherin interaction across cells
Communications Biology, 5, 1065, 2022.10
Tran Q, Osabe K, Entani T, Wazawa T, Hattori M, Nagai T
Application of Green enhanced Nano-lantern as a bioluminescent ratiometric indicator for measurement of Arabidopsis thaliana root apoplastic fluid pH
Plant, Cell & Environment, 45, 3157-3170, 2022.07
Lu K, Wazawa T, Sakamoto J, Vu C.Q, Nakano M, Kamei Y, Nagai T
Intracellular heat transfer and thermal property revealed by kilohertz temperature imaging with a genetically encoded nanothermometer
Nano Letters, 22, 5698, 2022.06
Sato S, Ohzawa S, Sota K, Sakamoto N, Udo A, Sueda S, Masuda T, Nagai T and Takenaka S
Fluorescence imaging of extracellular potassium ion using potassium sensing oligonucleotide
Frontier in Chemistry, 11, 2022.05
Zhai L, Nakashima R, Shinoda H, Ike Y, Matsuda T and Nagai T
Structure-based analysis and evolution of a monomerized red-colored chromoprotein from the Olindias formosa jellyfish
Protein Science, 31, e4285, 2022.05
[ Books ]
永井 健治 他
センチメートル規模の視野をマイクロメートルレベルの空間分解能でワンショット観察可能なトランススケールスコープ
診療における薬理遺伝学検査の社会実装に向けて, 医歯薬出版, 医学のあゆみ, 283, 7:728-729, 2022.11.12
永井健治, 松田知己, 鈴木和志, 篠田肇
タンパク質プローブ
先端の分析法 (監修：澤田嗣郎）, NTS, 317-323, 2022.01.29
服部満，永井健治
生物発光顕微鏡
先端の分析法 (監修：澤田嗣郎）, NTS, 397-400, 2022.01.29
Horikawa K and Nagai T
Live Imaging of cAMP Signaling in D. discoideum Based on a Bioluminescent Indicator, Nano-lantern (cAMP)
Methods Molecular Biology, 2483:231-240, 2022
Hattori M, Itoh Y, and Nagai T
Method for measuring bioactive molecules in blood by a smartphone using bioluminescent ratiometric indicators
Methods Molecular Biology, 2525:219-226, 2022
[ Reviews ]
Nagai T, Hattori M
Tiny but bright
nature reviews chemistry, 2022
[ Reviews in Japanese ]
永井健治, 市村垂生
100万以上の細胞をミクロンレベルの空間分解能でワンショット観察可能なトランススケールスコープAMATERAS－外れ値科学の創出をめざして－
シン・マクロファージ（編集：佐藤壮）, 羊土社, 実験医学, 40, 186-192, 2022
市村 垂生, 永井 健治
100万以上の細胞を同時観察可能なトランススケールスコープ
日本工業出版，画像ラボ, 33, 17-23, 2022.04.10
松田 知己, 服部 満, 永井 健治
蛍光・生物発光タンパク質を利用した機能分子の開発
材料の科学と工学, 日本材料科学会, 59, 42-45, 2022.04.20
[ Original papers ]
Goto A, Bota A, Miya K, Wang J, Tsukamoto S, Jiang X, Hirai D, Murayama M, Matsuda T, McHugh TJ, Nagai T and Hayashi Y
Stepwise synaptic plasticity events drive the early phase of memory consolidation
Science, 374, 857, 2021 [Press release]
Kanadome T, Hoshino N, Nagai T, Matsuda T, Yagi T
Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin
Scientific Reports, 11, 22237, 2021
Hattori M, Sugiura N, Wazawa T, Matsuda T and Nagai T
Ratiometric bioluminescent indicator for a simple and rapid measurement of thrombin activity using a smartphone
Analytical Chemistry, 93, 13520, 2021 [ Cover art ]
Kubo T, Temma K, Sugiura K, Shinoda H, Lu K, Smith NI, Matsuda T, Nagai T and Fujita K
Visible-wavelength multiphoton activation confocal microscopy
ACS Photonics, 8, 2666-2673, 2021
Ichimura T, Kakizuka T, Horikawa K, Seiriki K, Kasai A, Hashimoto H, Fujita K, Watanabe TM and Nagai T
Exploring rare cellular activity in more than one million cells by a transscale scope
Scientific Reports, 11, 16539, 2021
Vu C.Q, Fukushima S, Wazawa T and Nagai T
A highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like polypeptide
Scientific Reports, 11, 16519, 2021
Kaku T, Sugiura K, Entani T, Osabe K and Nagai T
Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer
Scientific Reports, 11, 14994, 2021
Tran Q, Osabe K, Entani T and Nagai T
A novel petal up-regulated PhXTH7 promoter analysis in Petunia hybrida by using bioluminescence reporter gene
Plant Biotechnology, 38, 197-204, 2021
Wazawa T, Noma R, Uto S, Sugiura K, Washio T and Nagai T
A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging
Microscopy, 70, 340-352, 2021 [ Cover art ]
Itoh Y*, Hattori M*, Wazawa T, Arai Y and Nagai T (*co-first authors)
Ratiometric bioluminescent indicator for simple and rapid diagnosis of bilirubin
ACS Sensors, 6, 889-895, 2021
Kubo T, Temma K, Smith NI, Lu K, Matsuda T, Nagai T and Fujita K
Hyperspectral two-photon excitation microscopy using visible wavelength
Optics Letters, 46, 37-40, 2021
[ Preprint ]
Kanadome T, Hoshino N, Nagai T, Matsuda T, Yagi T
Development of FRET-based indicators For visualizing homophilic trans interaction of a clustered protocadherin
Research Square, 2021
[ Books ]
Suzuki K, Hossain M.N, Matsuda T and Nagai T
Multicolor Bioluminescence Imaging of Subcellular Structures and Multicolor Calcium Imaging in Single Living Cells
Multiplexed Imaging, 229-237, Springer Nature, 2021
Hattori M, Matsuda T and Nagai T
Method for detecting emission spectral change of bioluminescent ratiometric indicators by a smartphone
Live Cell Imaging, ( Edited by Sung-Bae Kim) 295-304, Springer Nature, 2021
Riani Y, Matsuda T and Nagai T
Genetically Encoded Photosensitizer for Destruction of Protein or Cell Function
Optogenetics, (Edited by Yawo H, Kandori H, Koizumi A, Kageyama R), 265-279, Springer Nature, 2021
杉浦 一徳, 永井 健治
バイオイメージングのための蛍光タンパク質改変法
細胞組織化学2021 -生体分子の機能・局在を正しく捉えるための組織細胞化学の基礎と応用- 日本組織細胞化学会 編, 175-183, 2021
[ 解説記事 ]
永井 健治，長部 謙二
LEPによる持続可能社会の実現 -電気がなくても世界を照らす-
けいはんなView, vol.51, 6p, 2021.12
[ Others ]
永井 健治
2021年日本学士院賞：宮脇敦史博士の業績紹介
現代化学, 604, 75-76, 2021
野間 涼平, 和沢 鉄一, 宇上 周作, 杉浦 一徳, 鷲尾 隆, 永井 健治
表紙：光スイッチング蛍光タンパク質(Kohinoor2.0)による、長時間・高速超解像イメージング
日本バイオイメージング学会, 30, 1-2, 2021 [ Cover art ]
[ Original papers ]
Hattori M, Shirane S, Matsuda T, Nagayama K and Nagai T
Smartphone-based portable bioluminescence imaging system enabling observation at various scales from whole mouse body to organelle
Sensors, 20, 7166, 2020
Harada T, Sada R, Osugi Y, Matsumoto S, Matsuda T, Hayashi Nishino M, Nagai T, Harada A and Kikuchi A
Palmitoylated CKAP4 regulates mitochondrial functions through an interaction with VDAC2 at ER-mitochondria contact sites
Journal of Cell Science, 133, jcs249045, 2020
Nakamura S, Shigeyama S, Minami S, Shima T, Akayama S, Matsuda T, Esposito A, Napolitano G, Kuma A, Namba-Hamano T, Nakamura J, Yamamoto K, Sasai M, Tokumura A, Miyamoto M, Oe Y, Fujita T, Terawaki S, Takahashi A, Hamasaki M,
Yamamoto M, Okada Y, Komatsu M, Nagai T, Takabatake Y, Xu H, Isaka Y, Ballabio A and Yoshimori T
LC3 lipidation is essential for TFEB activation during the lysosomal damage response to kidney injury
Nature Cell Biology, 22, 1252–1263, 2020
Hossain M.N, Ishida R, Hattori M, Matsuda T and Nagai T
Bioluminescent Ratiometric Indicator for Analysis of Water Hardness in Household Water
Sensors, 20, 3164, 2020
Kuboki T, Ebata H, Matsuda T, Arai Y, Nagai T and Kidoaki S
Hierarchical development of motile polarity in durotactic cells just crossing an elasticity boundary
Cell Structure and Function, 45, 33-43, 2020
Araki S, Nakano M, Tsugane M, Sunaga F, Hattori M, Nagai T and Suzuki H.
A simple microfluidic device for live-imaging of the vertical section of epithelial cells
Analyst, 145, 667-674, 2020
[ Preprint ]
Ichimura T, Kakizuka T, Horikawa K, Seiriki K, Kasai A, Hashimoto H, Fujita K, Watanabe M.T and Nagai T
Trans-scale-scope to find rare cellular activity in sub-million cells
bioRχiv, 2020
Kakizuka T, Hara Y, Ohta Y, Mukai A, Ichiraku A, Arai Y, Ichimura T, Nagai T and Horikawa K
Cellular logics bringing the symmetry breaking in spiral nucleation revealed by trans-scale imaging
bioRχiv, 2020
[ Reviews ]
Nagai T, Chikuma S and Hanaoka K
Detection of singularity in immunity and cancer by novel imaging techniques
Biophysics and Physicobiology, 17, 98-99, 2020
Wazawa T, Washio T and Nagai T
Highly Biocompatible Super-resolution Imaging: SPoD-OnSPAN
Single Molecule Microscopy, Yamamoto, N. & Okada, Y. Eds., Neuromethods, 229-244, 2020
[ Proceedings ]
Kubo T, Temma K, Smith N. I, Kai L, Matsuda T, Nagai T and Fujita K
Hyperspectral fluorescence imaging by using visible-wavelength two-photon excitation
SPIE Proceedings 11521, 1152117, 2020
[ Reviews in Japanese ]
坂本 丞, 永井 健治, 亀井 保博
蛍光タンパク質温度プローブとその活用
細胞, 52, 14, 41-45, 2020
服部 満, 松田 知己, 永井 健治
バイオセンサ
炎症と免疫, 28, 71-74, 2020
篠田 肇
耐酸性可逆的光スイッチ緑色蛍光タンパク質
生物物理, 60, 176-177, 2020
松田知己, 永井健治
多色の光増感蛍光タンパク質を用いた複数機能の個別破壊
アグリバイオ, 4, 61-65, 2020
松田 知己, 永井 健治
光増感蛍光タンパク質を用いた多色機能破壊
細胞, 52, 47-50, 2020
永井 健治, 稲垣 成矩, 揚妻 正和
自由行動している複数マウスの脳活動を実時間計測する新たな技術－社会性行動を司さどる脳機能研究の新たなる展開について－
自動車技術, 74, 100-101, 2020
松田 知己, 永井 健治
高輝度発光タンパク質による高感度バイオイメージング
光学, 49, 26-30, 2020
[ Books ]
永井 健治
光るタンパク質が拓く未来社会
北海道大学公共政策大学院ブックレット, HPOS文理融合ルネッサンス -先端的政策課題のゆくえ- 遠藤乾・鈴木一人（編）, 第6章, 95-107
[ Others ]
永井 健治
追想：第57回“お祭り”年会＠宮崎
生物物理, 60, 192-193, 2020
[ Original papers ]
Shinoda H, Lu Kai, Nakashima R, Wazawa T, Noguchi K, Matsuda T and Nagai T
Acid-Tolerant Reversibly Switchable Green Fluorescent Protein for Super-resolution Imaging under Acidic Conditions
Cell Chemical Biology, 26, 1469-1479, 2019
Farhana I, Hossain M.N, Suzuki K, Matsuda T and Nagai T
Genetically Encoded Fluorescence/Bioluminescence Bimodal Indicators for Ca2+ Imaging
ACS Sensors, 4, 1825-1834, 2019
Oketani R, Suda H, Uegaki K, Kubo T, Matsuda T, Yamanaka M, Arai Y, Smith N, Nagai T and Fujita K
Visible-wavelength two-photon excitation microscopy with multifocus scanning for volumetric live-cell imaging
Journal of Biomedical Optics, 25, 014502, 2019
Inagaki S, Agetsuma M, Ohara S, Iijima T, Yokota H, Wazawa T, Arai Y and Nagai T
Imaging local brain activity of multiple freely moving mice sharing the same environment
Scientific Reports, 9, 7460, 2019
[ Reviews ]
Lu K, Vu CQ, Matsuda T and Nagai T
Fluorescent protein-based indicators for functional super-resolution imaging of biomolecular activities in living cells
Int. J. Mol. Sci, 20, 5784, 2019
[ Reviews in Japanese ]
前島 一博, 松田 知己, 永井 健治
蛍光マグネシウムイオンプローブの開発とそれを利用した新たな染色体凝縮メカニズムの解明
マグネシウム（Journal of Japanese society for Magnesium Research）, 37, 13-18, 2019
鈴木 和志, 稲垣 成矩, 松田 知己, 永井 健治
細胞・個体イメージング用光学プローブの開発
化学工業, 70, 516-520, 2019
和沢 鉄一, 鷲尾 隆, 永井 健治
回転偏光照明蛍光顕微鏡と光スイッチング蛍光タンパク質を用いた超解像イメージング法：SPoD-OnSPAN
光アライアンス, 30, 8, 15-19, 2019
[ Books ]
永井 健治, 篠田 肇
細胞内酸性環境の理解に向けた，耐酸性GFPの開発と応用
細胞組織化学2019 -組織細胞化学の基礎と応用：分子を観る，細胞を知る，機能を探る- 日本組織細胞化学会 編, 199-207, 2019
永井 健治，小澤 岳昌(編)
最強のステップUPシリーズ発光イメージング実験ガイド
羊土社，2019
[ Original papers ]
Nezu A, Morita T, Nagai T and Tanimura A
Simultaneous monitoring of Ca2+ responses and salivary secretion in live animals reveals a threshold intracellular Ca2+ concentration for salivation
The Physiological Society, 104, 61-69, 2018
Arai Y, Takauchi H, Ogami Y, Fujiwara S, Nakano M, Matsuda T and Nagai T
Spontaneously blinking fluorescent protein for simple single laser super-resolution live cell imaging
ACS Chemical Biology, 13, 1938-1943, 2018
Komatsu N, Terai K, Imanishi A, Kamioka Y, Sumiyama K, Jin T, Okada Y, Nagai T and Matsuda M
A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging
Scientific Reports, 8, 8984, 2018
Sato R, Kawashima R, Mai Duy Luu T, Nakano M, Nagai T and Masuda S
Significance of PGR5-dependent cyclic electron flow for optimizing the rate of ATP synthesis and consumption in Arabidopsis chloroplasts
Photosynthesis Research, 139, 359-365, 2018
Suzuki K*, Onishi T*, Nakada C, Takei S, J. Daniels M, Nakano M, Matsuda T and Nagai T
Uninterrupted monitoring of drug effects in human-induced pluripotent stem cell-derived cardiomyocytes with bioluminescence Ca2+ microscopy
BMC Research Notes, 11, 313, 2018 (* equal contribution)
Riani Y D, Matsuda T, Takemoto K, Nagai T
Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation
BMC Biol, 16, 2018
Hara-Kuge S, Nishihara T, Matsuda T, Kitazono T, Teramoto T, Nagai T and Ishihara T
An improved inverse-type Ca2+ indicator can detect putative neuronal inhibition in Caenorhabditis elegans by increasing signal intensity upon Ca2+ decrease
PLoS ONE, 13, e0194707,2018
Hossain M.N, Suzuki K, Iwano M, Matsuda T and Nagai T
Bioluminescent low-affinity Ca2+ indicator for ER with multicolor calcium imaging in single living cells
ACS Chemical Biology, 13, 1862-1871, 2018
Ohta Y, Furuta T, Nagai T and Horikawa K
Red fluorescent cAMP indicator with increased affinity and expanded dynamic range
Sci. Rep., 8, 1866, 2018
Wazawa T, Arai Y, Kawahara Y, Takauchi H, Washio T, and Nagai T
Highly biocompatible super-resolution fluorescence imaging using the fast photoswitching fluorescent protein Kohinoor and SPoD-ExPAN with Lp-regularized image reconstruction
Microscopy, 67, 89-98, 2018
Kushida Y, Arai Y, Shimono K, and Nagai T
Biomimetic chemical sensing by fluorescence signals using a virus-like particle-based platform
ACS Sensors, 3, 87-92, 2018
Maeshima K*, Matsuda T*, Shindo Y*, Imamura H*, Tamura S, Imai R, Kawakami S, Nagashima R, Soga T, Noji H, Oka K and Nagai T
A transient rise in free Mg2+ ions released from ATP-Mg by its hydrolysis contributes to mitotic chromosome condensation
Current Biology, 28, 444-451.e6, 2018 (* equal contribution)
Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T
Acid-tolerant monomeric GFP from Olindias formosa
Cell Chemical Biology, 25, 330-338, 2018
[ Reviews ]
Shinoda H, Michael S and Nagai T
Fluorescent proteins for investigating biological events in acidic environments
International Journal of Molecular Science, 19, 1548, 2018
[ Books ]
永井 健治, 鈴木 和志, 稲垣 成矩
化学発光ライブイメージングの現状と展望
細胞組織化学2018 -組織細胞化学技法の基礎から最先端まで：形を観て，機能を識る- 日本組織細胞化学会 編, 185-193, 2018
原田 慶恵，永井 健治(編)
生きてるものは全部観る！ イメージングの選び方・使い方100＋
羊土社 実験医学増刊, 36, 2018
Editors: Nagai T, Togashi Y
Minorities and Small Numbers from Molecules to Organisms in Biology Toward a New Understanding of Biological Phenomena Springer
Springer Singapore, 2018
Nagai T, Riani YD
Optogenetic control of generation of reactive oxygen species for photo-inducible protein inactivation and cell ablation
Optogenetics, Royal Society of Chemistry, Accepted
Nagai T, Horikawa K, Saito K, Matsuda T
Genetically encoded Ca2+ indicators; expanded affinity range, color hue and compatibility with optogenetics
Application of Genetically Encoded Indicators to Mammalian Central Nervous System, Frontiers in Molecular Neuroscience, 38-42
[ Original papers ]
Toba S, Jin M, Yamada M, Kumamoto K, Matsumoto S, Yasunaga T, Fukunaga Y, Miyazawa A, Fujita S, Itoh K, Fushiki S, Hiroaki K, Wanibuchi H, Arai Y, Nagai T, and Hirotsune S
Alpha-synuclein facilitates to form short unconventional microtubules that have a unique function in the axonal transport
Sci. Rep., 7, 16386, 2017
Nozaki T, Imai R, Tanbo M, Nagashima R, Tamura S, Tani T, Joti Y, Tomita M, Hibino K, Kanemaki MT, Wendt KS, Okada Y, Nagai T, and Maeshima K
Dynamic organization of chromatin domains revealed by superresolution live-cell imaging
Molecular Cell, 67, 282-293, 2017
Aoyama M, *Yoshioka Y, Arai Y, Hirai H, Ishimoto R, Nagano K, Higashisaka K, Nagai T, *Tsutsumi Y
Intracellular trafficking of particles inside endosomal vesicles is regulated by particle size
Journal of Controlled Release, 260, 183-193, 2017
Seiriki K, Kasai A, Hashimoto T, Schulze W, Niu M, Yamaguchi S, Nakazawa T, Inoue K, Uezono S, Takada M, Naka Y, Igarashi H, Tanuma M, Waschek JA, Ago Y, Tanaka KF, Hayata-Takano A, Nagayasu K, Shintani N, Hashimoto R,
Kunii Y, Hino M, Matsumoto J, Yabe H, Nagai T, Fujita K, Matsuda T, Takuma K, Baba A, and Hashimoto H
High-speed and scalable whole-brain imaging in rodents and primates
Neuron, 94, 1085-1100, 2017
Matsushita J*, Inagaki S*, Nishie T, Sakasai T, Tanaka J, Watanabe C, Mizutani K, Miwa Y, Matsumoto K,
Takara K, Naito H, Kidoya H, Takakura N, Nagai T, Takahashi S and Ema M (*: co-first author)
Fluorescence and bioluminescence imaging of angiogenesis in Flk1-Nano-lantern transgenic mice
Sci. Rep., 7, 46597, 2017
Chang YF, Broyles CN, Brook F, Davies MJ, Turtle C, Nagai T and Daniels MJ
Non-invasive phenotyping and drug testing in single cardiomyocytes or beta-cells by calcium imaging and optogenetics
PLoS ONE, 12, e0174181, 2017
Nakano M, Arai Y, Kotera I, Okabe K, Kamei Y and Nagai T
Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response
PLoS ONE, 12, e0172344, 2017
Suzuki M, Sato M, Koyama H, Hara Y, Hayashi K, Yasue N, Imamura H, Fujimori T, Nagai T, E. Campbell R, and Ueno N
Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure
Development, 144, 1307-1316, 2017
Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G,J. Daniels M, Okamura Y, Matsuda T, and Nagai T
Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging
Scientific Reports, 7, 42398, 2017
Takemoto K, Iwanari H, Tada H, Suyama K, Sano A, Nagai T, Hamakubo T, and Takahashi T
Optical inactivation of synaptic AMPA receptors erases fear memory
Nature Biotechnology, 35, 38-47, 2017
Kim S, Arai Y, Tani T, Takatsuka H, Saito Y, Kawashima T, Kawakami S, Miyawaki A, and Nagai T
Simultaneous imaging of multiple cellular events using high-accuracy fluorescence polarization microscopy
Microscopy, 66, 110-119, 2017
[ Proceedings ]
和沢鉄一，新井由之，河原吉伸，中野雅裕，鷲尾隆，永井健治
遺伝子工学的に開発した蛍光プローブによる細胞生理機能超解像イメージング
The 31st Annual Conference of the Japanese Society for Artificial Intelligence, 2017
Inagaki S, and Nagai T
Bioluminescent indicator applicable to membrane voltage recording in various excitable cell types
SPIE Proceedings10251, 1025102, 2017
[ Reviews ]
Suzuki K and Nagai T
Recent progress in expanding the chemiluminescent toolbox for bioimaging
Current Opinion in Biotechnology, 48, 135-141, 2017
Nakano M, Nagai T
Thermometers for monitoring cellular temperature
Journal of Photochemistry and Photobiology, C: Photochemistry Reviews, 30, 2-9, 2017
Agetsuma M, Matsuda T, and Nagai T
Methods for monitoring signaling molecules in cellular compartments
Cell Calcium, 64, 12-19, 2017
[ Books ]
永井健治, 冨樫祐一(編)
少数性生物学
日本評論社, 2017
[ Reviews in Japanese ]
永井健治
1個と無限個の狭間に潜む新パラダイム
実験医学, 35, 3184-3189, 2017
堀川一樹，太田裕作，向井あすか，新井由之，永井健治
細胞集団シグナル伝達の少数制御
実験医学, 35, 3204-3210, 2017
鈴木和志，永井健治
高光度化学発光タンパク質の開発と生命科学研究への応用
生物物理, 57, 262-264, 2017
中野雅裕，永井健治
高輝度化学発光タンパク質の開発
生体の科学, 68(5), 462-463, 2017
和沢鉄一，新井由之，永井健治
SPoD-ExPAN超解像イメージング
顕微鏡, 77-81, 2017
[ Preprint server ]
Inagaki S, Agetsuma M, Ohara S, Iijima T, Wazawa T, Arai Y, Nagai T
In vivo brain activity imaging of interactively locomoting mice
bioRxiv, Posted October 15, 2017
Arai Y, Takauchi H, Ogami Y, Fujiwara S, Nakano M, Nagai T
A spontaneously blinking fluorescent protein for simple single laser super-resolution live cell imaging
bioRxiv, Posted October 8, 2017
[ Original papers ]
Shimada N, Saito M, Shukuri S, Kuroyanagi S, Kuboki T, Kidoaki S, Nagai T, and Maruyama A
Reversible monolayer/spheroid cell culture switching by UCST-type thermoresponsive ureido polymers
ACS Applied Materials & Interfaces, 8, 31524 - 31529, 2016
Suzuki K, Kimura T, Shinoda H, Bai G, Daniels M, Arai Y, Nakano M, and Nagai T
Five color variants of bright luminescent protein for real-time multicolor bioimaging
Nature Communications, 7, 13718, 2016
読売新聞、日刊工業新聞、NHKニュースなどで報道（報道数186件）
Lu-Walther H, Hou W, Kielhorn M, Arai Y, Nagai T, Kessels M, Qualmann B, and Heintzmann R
Nonlinear structured illumination using a fluorescent protein activating at the readout wavelength
PLoS ONE, 11, e0165148, 2016
Mochida S, Rata Scott, Hino H, Nagai T,and Novak B
Two bistable swithes govern M phase entry
Current Biology, 26, 3361-3367, 2016
Sakamaki K, Ishii MT, Sakata T, Takemoto K, Takagi C, Takeuchi A, Morishita R, Takahashi H, Nozawa A, Shinoda H, Chiba K, Sugimoto H, Saito A, Tamate S, Satou Y, Sang-Kee J, Matsuoka S, Koyamada K, Sawasaki T, Nagai T,and Ueno N
Dysregulation of a potassium channel, THIK-1, targeted by caspase-8 accelerates cell shrinkage
Biochim. Biophys. Acta Mol. Cell Res., 1863, 2766-2783, 2016
Ohta Y, Kamagata T, Mukai A, Takada S, Nagai T,and Horikawa K
Non-trivial effect of the color-exchange of a Donor/Acceptor pair in the engineering of Forster resonance energy transfer (FRET)-based indicators
ACS Chemical Biology, 11, 1816-1822, 2016
Pandey K, Ferreira PE, Ishikawa T, Nagai T, Kaneko O, and Yahata K
Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor
Scientific Reports 6, 23454, 2016
Lissek T, Obenhaus HA , Ditzel DAW, Nagai T, Miyawaki A , Sprengel R, and Hasan MT
General anesthetic conditions induce network synchrony and disrupt sensory processing in the cortex
Frontiers in Cellular Neuroscience, 4, 16, 2016
Morikawa TJ, Fujita H, Kitamura A, Horio T, Yamamoto J, Kinjo M, Sasaki A, Machiyama H, Yoshizawa K, Ichimura T, Imada K, Nagai T, and Watanabe TM
Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it
Scientific Reports, 6, 22342, 2016
[ Reviews ]
Inagaki S, and Nagai T
Current progress in genetically encoded voltage indicators for neural activity recording
Current Opinion In Chemical Biology, 33, 95-100, 2016
[ Reviews in Japanese ]
稲垣成矩，永井健治
遺伝子にコードされた膜電位センサーによる神経活動計測の現状と展望
Drug Delivery System, 31-2, 119-126, 2016
[ Original papers ]
Wazawa T, Morimoto, Nagai T. and Suzuki M
Rotational motion of rhodamine 6G tethered to actin through oligo(ethylene glycol) linkers studied by frequency-domain fluorescence anisotropy
Biophysics and Physicobiology, 12, 87-102, 2017
Hanasaki I, Uehara S, Arai Y, Nagai T and Kawano S
Threshold-free evaluation of near-surface diffusion and adsorption-dominated motion from single-molecule tracking data of single-stranded DNA through total internal reflection fluorescence microscopy
Jpn. J. Appl. Phys, 54, 125601, 2015
Iwano M, Ito K, Fujii S, Kakita M, Asano-Shimosato H, Igarashi M, Kaothien-Nakayama P, Entani T, Kanatani A, Takahisa M, Tanaka M, Komatsu K, Shiba H, Nagai T, Miyawaki A, Isogai A and Takayama S
Calcium signalling mediates self-incompatibility response in the Brassicaceae
Nature Plants, 1, 15128, 2015
Kim K, Lakhanpal G, Lu HE, Khan M, Suzuki A, Kato-Hayashi M, Narayanan R, Luyben TT, Matsuda T, Nagai T, Blanpied TA, Hayashi Y, and Okamoto K
A Temporary Gating of Actin Remodeling during Synaptic Plasticity Consists of the Interplay between the Kinase and Structural Functions of CaMKII
Neuron, 87, 813-826, 2015
Vadim PK, Matsuda T, Nagai T
MagIC, a genetically encoded fluorescent indicator for monitoring cellular Mg2+ using a non-FRET ratiometric imaging approach
Journal of Biomedical Optics, 20, 101203, 2015
Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, Fujita K and Nagai T
A fast- and positively photoswitchable fluorescent protein for ultralow-laser-power RESOLFT nanoscopy
Nature Methods, 12, 515-518, 2015 （日刊工業新聞, 産経WEST掲載）
Nezu A, Morita T,Tojyo Y, Nagai T and Tanimura A
Partial agonistic effects of pilocarpine on Ca2+ responses and salivary secretion in the submandibular glands of live animals
Experimental Physiology, 100, 640-651, 2015
Arai Y, Yamamoto T, Minamikawa T, Takamatsu T and Nagai T
Spectral fingerprinting of individual cells visualized by cavity-reflection-enhanced light-absorption microscopy
PLoS ONE, 10, e0125733, 2015
Yamanaka M, Saito K, Smith IN, Arai Y, Uegaki K, Yonemaru Y, Mochizuki K, Kawata S, Nagai T and Fujita K
Visible-wavelength two-photon excitation microscopy for fluorescent protein imaging
Journal of Biomedical Optics, 20, 101202, 2015
Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin T, Okada Y and Nagai T
Expanded palette of Nano-lantern for real-time muliti-color luminescence imaging
Proc. Natl. Acad. Sci. USA, 112, 4352-4356, 2015 (NHK 朝の関西ニュースにて放映、産経新聞・時事通信社・日刊工業新聞・日本経済新聞・毎日新聞に掲載)
Ichijo H, Hamada M, Takahashi S, Kobayashi M, Nagai T, Toyama T, Kawaguchi M
Lateralization, maturation, and anteroposterior topography in the lateral habenula revealed by ZIF268/EGR1 immunoreactivity and labeling history of neuronal activity
Neuroscience Reserach, 95, 27-37, 2015
Sugiura K, Nagai T, Nakano M, Ichinose H, Nakabayashi T, Ohta N, Hisabori T
Redox sensor proteins for highly sensitive direct imaging of intracellular redox state
Biochemical and Biophysical Research Communications, 457, 242-248, 2015
[ Reviews ]
Uno S, Tiwari DK, Kamiya M, Arai Y, Nagai T, Urano Y
A guide to use photocontrollable fluorescent proteins and synthetic smart fluorophores for nanoscopy
Microscopy, 64, 4, 263-277, 2015
Saito K, Nagai T
Recent progress in luminescent proteins development.
Current Opinion In Chemical Biology, 27, 46-51, 2015
Nagai T
Real time imaging of biological phenomena with super-duper luminescent proteins
CYTOLOGIA, 80, 1-2, 2015
[ Reviews in Japanese ]
中野雅裕，永井健治
肉眼でも観察できる！3色（水色, 黄緑色, 橙色）の高光度発光タンパク質
O plus E, 428, 505-506, 2015
[ Original papers ]
Oshima Y, Imamura T, Shintani A, Kajiura-Kobayashi H, Hibi T, Nagai T, Nonaka S, Nemoto T
Ultrasensitive imaging of Ca2+ dynamics in pancreatic acinar cells of Yellow Cameleon-Nano transgenic mice
International Journal of Molecular Sciences, 15, 19971-19986, 2014
Yoshiyama S, Chen Z, Okagaki T, Kohama K, Nasu-Kawaharada R, Izumi T, Ohshima N, Nagai T, Nakamura A
Nicotine exposure alters human vascular smooth muscle cell phenotype from a contractile to a synthetic type
Atherosclerosis, 237, 464-470, 2014
Jin M, Yamada M, Arai Y, Nagai T, Hirotsune S
Arl3 and LC8 regulate dissociation of dynactin from dynein
Nature Communications, 24, 5295, 2014
Kanemaru K, Sekiya H, Xu M, Satoh K, Kitajima N, Yoshida K, Okubo Y, Sasaki T, Moritoh S, Hasuwa H, Mimura M, Horikawa K, Matsui K, Nagai T, Iino M, Tanaka KF
In vivo visualization of subtle, transient and local activity of astrocytes using an ultrasensitive Ca2+ indicator
Cell Reports, 8, 311-318, 2014
Uehara S, Hanasaki I, Arai Y, Nagai T, Kawano S
Statistical characterisation of single-stranded DNA motion near glass surface beyond diffusion coefficient
Micro and Nano Letters, 9, 257-260, 2014
Fukuda N, Matsuda T, Nagai T
Optical control of the Ca2+ concentration in a live specimen with a genetically encoded Ca2+-releasing molecular tool
ACS Chemical biology, 9, 1197-1203, 2014
[ Reviews ]
Nagai T, Horikawa K, Saito K, Matsuda T
Genetically encoded Ca2+ indicators; expanded affinity range, color hue and compatibility with optogenetics
Frontiers in Neuroscience, 7, 25, 2014
Arai Y, Nagai T
Real-Time chemiluminescence imaging using Nano-Lantern probes
Current Protocols in Chemical Biology, 6, 221-236
Matsuda T, Nagai T
Quantitative measurement of intracellular protein dynamics using photobleaching or photoactivation of fluorescent proteins
Microscopy, 63, 403-408, 2014
[ Reviews in Japanese ]
永井健治
回折限界を超えた超解像蛍光顕微鏡
化学, 69, 21-26, 2014
松田知己，永井健治
光スイッチング機能プローブで挑む細胞の個性
生体の科学, 65, 101-106, 2014
齋藤健太，永井健治
高輝度化学発光タンパク質Nano-lanternの開発
化学と生物, 52, 646-650, 2014
永井健治
今のめり込み術のマルチ効能
生物物理, 54, 75, 2014
永井健治，松田知己
蛍光タンパク質が拓く超解像技術
化学フロンティア, 1分子ナノバイオ計測 15章
新井由之，永井健治
CCDカメラのデッドタイムを利用した光刺激法が可能にする蛍光・化学発光イメージングとオプトジェネティクスの高時間分解併用
生化学, 86, 2014
[ Original papers ]
Takemoto K, Matsuda T, Sakai N, Fu D, Noda M, Uchiyama S, Kotera I, Arai Y, Horiuchi M, Fukui K, Ayabe T, Inagaki F, Suzuki H, Nagai T
SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
Scientific Reports, 3, 2629, 2013
Nozaki T, Kaizu K, Pack C, Tamura S, Tani T, Hihara S, Nagai T, Takahashi K, Maeshima K
Flexible and dynamic nucleosome fiber in living mammalian cells
Nucleus, 4, 1-8, 2013
Yamanaka M, Saito K, Smith NI, Kawata S, Nagai T, Fujita K
Saturated excitation (SAX) of fluorescent proteins for sub-diffraction-limited imaging of living cells in three dimensions
Interface Focus, 3, 20130007, 2013
Yamada M, Kumamoto K, Mikuni S, Arai Y, Kinjo M, Nagai T, Tsukasaki Y, Watanabe TM, Fukui M, Jin M, Toba S, Hirotsune S
Rab6a releases LIS1 from a dynein idling complex and activates dynein for retrograde movement
Nature Communications, 4, 2033, 2013
Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang Y-F, Araki S, Arai Y, March K, Thomas H, Sagou K, Miyata T, Nagai T, Li W, Campbell R
Improved orange and red Ca2+ indicators and photophysical considerations for optogenetic applications
ACS Chemical Neuroscience, 4, 963-972, 2013
Matsuda T, Horikawa K, Saito K, Nagai T
Highlighted Ca2+ imaging with a genetically encoded 'caged' indicator
Scientific Reports, 3, 1398, 2013
Saito K, Higuchi Y, Arai Y, and Nagai T
Video-rate imaging of luminescent tumour cells in freely moving unshaved mice
Protocol Exchange
Hoi H, Matsuda T, Nagai T, Campbell RE
Highlightable Ca2+ indicators for live cell imaging
JACS, 135, 46-49, 2013
[ Reviews ]
Arai.Y, and Nagai T
Extensive use of FRET in biological imaging
Microscopy, 62, 419–428, 2013
Tiwari D.K., and Nagai T
Smart fluorescent proteins: Innovation for barrier-free superresolution imaging in living cells
Development Growth and Differentiation, 55, 491-507, 2013
Koldenkova V.P. and Nagai T
Genetically encoded Ca2+ indicators: properties and evaluation
BBA - Molecular Cell Research, 1833, 1787-1797, 2013
[ Reviews in Japanese ]
永井健治，齋藤健太，初谷紀幸
化学発光タンパク質の高輝度化とバイオイメージングへの展開
顕微鏡, 48, 1-3, 2013
堀川一樹，永井健治
Ca2+イメージングで解き明かすCa2+動態の時空間統御機構
CLINICAL CALCIUM -循環器疾患とカルシウム・リン, 23, 2013
ビジュアルバイオロジー－タンパク質でできた蛍光Ca2+センサーの開発から－
生産と技術, 65, 2, 2013
[ Original papers ]
Hihara S, Pack CG, Hanafusa T, Kaizu K, Tani T, Takemoto S, Yoshimi T, Sako Y, Yokota H, Imamoto N, Kinjo M, Takahashi K, Nagai T, Maeshima K
Local nucleosome dynamics facilitate chromatin accessibility in living mammalian cells
Cell Reports, 2, 1645-56, 2012
Saito K, Chang Yu-Fen, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, Yoshida Y, Matsuda T, Arai Y, Nagai T
Luminescent protein for high-speed single-cell and whole-body imaging
Nature Communications, 3, 1262, 2012
Drug Discovery & Development誌にて当研究成果が紹介。Nature Methods，Nature Photonicsで紹介。Faculty of 1000にノミネート。
Kominami K, Nagai T, Sawasaki T, Tsujimura Y,Yashima K, Sunaga Y, Tsuchimochi M, Nishimura J, Chiba K, Nakabayashi J, Koyamada K, Endo Y, Yokota H, Miyawaki A, Manabe N, Sakamaki K
In vivo imaging of hierarchical spatiotemporal activation of caspase-8 during apoptosis
PLoS ONE, 7, e50218, 2012
Hatsugai N, Koldenkova VP, Imamura H, Noji H, Nagai T
Changes in cytosolic ATP levels and intracellular morphology during bacteria- induced hypersensitive cell death as revealed by real-time fluorescence microscopy imaging
Plant and Cell Physiology, 53, 1768-1775, 2012
Iwano M, Ngo QA, Entani T, Shiba H, Nagai T, Miyawaki A, Isogai A,Grossniklaus U, Takayama S
Cytoplasmic Ca2+ changes dynamically during the interaction of the pollen tube with synergid cells
Development, 139, 4202-4209, 2012
Kwon HJ, Ohmiya Y, Honma K-i, Honma S,Nagai T,Saito K, Yasuda K
Synchronized ATP oscillations have a critical role in prechondrogenic condensation during chondrogenesis
Cell Death and Disease, 3, e278, 2012
Kominami K, Nakabayashi J, Nagai T, Tsujimura Y, Chiba K, Kimura H, Miyawaki A, Sawasaki T, Yokota H, Manabe N, Sakamaki K
The molecular mechanism of apoptosis upon caspase-8 activation: Quantitative experimental validation of a mathematical model.
Biochim Biophys Acta-Molecular Cell Research, 1823, 1825-1840, 2012
Chang Y, Arai Y, Nagai T
Optogenetic activation during detector 'dead time' enables compatible real-time fluorescence imaging
Neuroscience Research, 73, 341-347, 2012
Ohtsuka K, Sato S, Sato Y, Sota K, Ohzawa S, Matsuda T, Takemoto K, Takamune N, Juskowiak B, Nagai T, Takenaka S T
Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer peptide conjugate
Chemical Communications, 48, 4740-4742, 2012
[ Reviews, Books ]
永井健治
おもろいバイオロジー "蛍光プローブ開発秘話その2～夏秋冬春～"
細胞工学, 31, 2012
[ Original papers ]
Saito K, Arai Y, Zhang J, Kobayashi K, Tani T, Nagai T
Conjugation of both on-axis and off-axis light in Nipkow disk confocal microscope to increase availability of incoherent light source
Cell Struct. Func., 36, 237-246, 2011
Yamada Y, Michikawa T, Hashimoto M, Horikawa K, Nagai T, Miyawaki A,Hausser M, Mikoshiba K
Quantitative comparison of genetically encoded Ca2+ indicators in cortical pyramidal cells and cerebellar Purkinje cells
Frontiers in Cellular Neuroscience, 5, 18, 2011
Zhao Y, Araki S, Wu J, Teramoto T, Chang Y, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
An expanded palette of genetically encoded Ca2+ indicators
Science, 333, 1888-1891, 2011
Nakano M, Imamura H, Nagai T, Noji H
Ca2+ regulation of mitochondrial ATP synthesis visualized at the single cell level
ACS Chemical Biology, 6, 709-15, 2011
Nomura M, Nagai T, Harada Y, Tani T
Facilitated intracellular transport of TrkA by an interaction with nerve growth factor
Developmental Neurobiology, 71, 634-649, 2011
Takemoto K, Matsuda T, McDougall M, Klaubert DH, Hasegawa A, Los GV, Wood KV, Miyawaki A, Nagai T
Chromophore-assisted light inactivation of HaloTag fusion proteins labeled with eosin in living cells
ACS Chemical Biology, 6, 401-406, 2011
Yang L, Matsuda T, Raviraj V, Ching YW, Braet F, Nagai T, Soon LL
Imaging the dynamics of intracellular protein translocation by photoconversion of Phamret-Cybr/ROM
Journal of Microscopy, 242, 250-261, 2011
[ Reviews, Books ]
齋藤 健太，永井 健治
生物発光を利用した細胞内カルシウムイメージング
生物物理, 52, 2011
永井 健治，堀川 一樹
機能イメージングにおける指示薬感度の重要性―蛍光タンパク質間FRETを用いたCa2+指示薬開発からの考察―
蛍光イメージング/MRIプローブの開発
CMC出版
堀川 一樹，永井 健治
細胞性粘菌の集合流形成における細胞間シグナル伝達
生物の科学, 遺伝 65, 87-91, 2011
斎藤 健太，永井 健治
化学発光を利用した生命機能の可視化
化学と生物, 49, 555-559, 2011
[ Original papers ]
Horikawa K, Yamada Y, Matsuda T, Kobayashi K, Hashimoto M, Matsu-ura T, Miyawaki A, Michikawa T, Mikoshiba K, Nagai T
Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano
Nature Methods, 7, 729-732, 2010
Saito K, Hatsugai N, Horikawa K, Kobayashi K, Matsu-Ura T, Mikoshiba K, Nagai T
Auto-luminescent genetically-encoded ratiometric indicator for real-time Ca2+ imaging at the single cell level
PLoS ONE, 5, e9935，2010
Lutcke H, Murayama M, Hahn T, Margolis DJ, Astori S, Borgloh SMzA, Gobel W, Yang Y, Tang W, Kugler S, Sprengel R, Nagai T, Miyawaki A, Larkum ME, Helmchen F, Hasan MT
Optical recording of neuronal activity with a genetically-encoded Ca2+ indicator in anesthetized and freely moving mice
Frontiers in Neural Circuit, 4, 1-12, 2010
Hong JH, Min CH, Jeong B, Kojiya T, Morioka E, Nagai T, Ikeda M. Lee K
Intracellular calcium spikes in rat suprachiasmatic nucleus neurons induced by BAPTA-based calcium dyes
PLoS ONE, 5, e9634，2010
Kotera I, Iwasaki T, Imamura H, Noji H, Nagai T
Reversible dimerization of Aequorea victoria fluorescent proteins increases the dynamic range of FRET-based indicators
ACS Chemical Biology, 5, 215-222, 2010
[ Reviews, Books ]
永井健治
なぜなぜ生物学
細胞の中って見えるの？
日本分子生物学会, 第十二章 153-167, 2010
永井健治
1枚の写真館 幼名と改名
細胞工学, 29, 843, 2010
永井健治
Dual FRET法を利用した複数の生理機能イメージング
シングルセル解析の最前線, 神原秀記, 松永是, 植田充美 監修 (分担執筆）
第一章 61-69, シーエムシー出版, 2010
永井健治
蛍光タンパク質エンジニアリングによるバイオイメージング技術の進展
レーザー研究, 38, 416-420, 2010
永井健治，小林健太郎
１波長励起Dual FRETによる生細胞内における複数現象の可視化
実験医学, 28, 589-598, 2010
[ Original papers ]
Iwano M, Entani T, Shiba H, Kakita H, Nagai T, Mizuno H, Miyawaki A, Shoji T, Kubo K, Isogai A, Takayama S
Fine-tuning of the cytoplasmic Ca2+ concentration is essential for pollen tube growth
Plant Physiology, 150, 1322-1334, 2009
Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa K, Nagai T
An ultramarine fluorescent protein with increased photostability and pH insensitivity
Nature Methods, 6, 351-353, 2009
Yamamoto T, Kumagai A, Saito K, Nagai T
Two-photon excitation behavior of thiophene-based oligomeres and a polymer
J Nanosci Nanotechnol., 9, 670-672, 2009
Imamura H, Nhat KPH, Togawa H, Saito K, Iino R, Kato-Yamada Y, Nagai T, Noji H
Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators
Proc. Natl, Aca, Sci. U.S.A., 106, 15651-15656, 2009
[ Reviews, Books ]
野馳博行，永井健治
生命システムの階層間をまたぐイメージング技術
蛋白質 核酸 酵素, 54, 1913-1917, 2009
堀川一樹，永井健治
細胞結合振動しシステムを対象にした超高感度大規模イメージング
蛋白質 核酸 酵素, 54, 1945-1951, 2009
永井健治，松田知己
生細胞内でのタンパク質動態測定とその意義
生物物理, 49, 181-186, 2009
永井健治，松田知己
光スイッチング蛍光タンパク質を用いた生細胞内でのタンパク質動態定量法
日本組織細胞化学会編, 組織細胞化学2009, 99-105, 2009
永井健治
酸性の環境でも光る蛍光タンパク質
化学, 64, 72-73, 2009
小寺一平，永井健治
ワンステップ全自動プラスミド構築方法
細胞工学, 28, 402-407, 2009
永井健治
光スイッチング蛍光タンパク質を用いた超解像観察法
分光研究, 58, 7-9, 2009
永井健治
蛍光プロープ開発秘話：Pericam
蛋白質 核酸 酵素, 54, 86-92, 2009
[ Original papers ]
Saito K, Kobayashi K, Tani T, Nagai T
A mercury arc lamp-based multi-color confocal real time imaging system for cellular structure and function
Cell Struct Funct. 33, 133-141, 2008
Kotera I, Nagai T
A high-throughput and single-tube recombination of crude PCR products using a DNA polymerase inhibitor and type IIS restriction enzyme
J. Biotechnol. 137, 1-7, 2008
Sunabori T, Tokunaga A, Nagai T, Sawamoto K, Okabe M, Miyawaki A, Matsuzaki Y, Miyata T, Okano H
Cell-cycle-specific nestin expression coordinates with morphological changes in embryonic cortical neural progenitors
J Cell Sci. 121, 1204-1212, 2008
Matsuda T, Miyawaki A, Nagai T
Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein
Nature Methods, 5, 339-345, 2008
[ Reviews, Books ]
永井健治，松田知己
光変換蛍光タンパク質を用いた生体分子の動態解析法
実験医学, 26, 156-162, 2008
松田知己，永井健治
新規の光変換蛍光蛋白質プローブを用いた生体イメージングと動態解析
蛋白質 核酸 酵素, 53, 1858-1864, 2008
松田知己，永井健治
光活性化・光変換蛍光タンパク質を用いたタンパク質動態解析
バイオインダストリー, 25, 21-26, 2008
[ Original papers ]
Takemoto K, Kuranaga E, Tonoki A, Nagai T, Miyawaki A, Miura M
Local initiation of caspase activation in Drosophila salivary gland programmed cell death in vivo
Proc Natl Acad Sci U S A., 104, 13367-13372, 2007
[ Reviews, Books ]
Wei C, Nagai T, Wei W, Nemoto T, Awais M, Niwa O, Kurita R, Baba Y
New advances in nanomedicine: diagnosis and preventive medicine
Med Clin North Am., 91, 871-879, 2007
永井健治, 改変型蛍光タンパク質の利用, 67-78
永井健治, 小寺一平, 共鳴エネルギー移動（FRET）の基礎, 110-117
永井健治, 斉藤健太, FRETの測定法と評価, 118-128
斉藤健太, 松田知己, 原口徳子, 永井健治, 実習6 FRET, 268-277
小寺一平, 谷知己, 永井健治, 実習9 全反射顕微鏡, 291-297
講義と実習 生細胞蛍光イメージング, 共立出版, 2007
竹本研, 永井健治，宮脇敦史，三浦正幸, アポトーシスの検出，143-146
谷知己，永井健治, Column：スパイはひとりで充分，147
小寺一平，永井健治, FRETプローブ，158-162
実験がうまくいく 蛍光・発光試薬の選び方と使い方，羊土社，2007
永井健治
蛍光イメージング─最近の進歩：回折限界の壁を越える工夫─
病理と臨床, 25, 514-519, 2007
齊藤健太，谷知己，小林健太郎，永井健治
大口径ファイバー白色光源を用いた高速共焦点顕微鏡の開発と評価
顕微鏡, 161-165, 2007
永井健治
発光性蛋白質を利用したバイオセンサーの開発
バイオとナノの融合 I 新生命科学の基礎, 北海道大学COE研究成果編集委員会, 第8章, 97-113, 北海道大学，2007
[ Original papers ]
Kazuno AA, Munakata K, Nagai T, Shimozono S, Tanaka M, Yoneda M, Kato N,
Miyawaki A, Kato T.
Identification of mitochondrial DNA polymorphisms that alter mitochondrial matrix pH and intracellular calcium dynamics
PLoS Genet. 2 , e128, 2006
[ Reviews, Books ]
永井健治
FRETの上手な使い方
蛋白質 核酸 酵素, 51, 1989-1997, 2006
永井健治
電顕に期待するもの 4
細胞工学, 25, 1192-1193, 2006
永井健治
HaloTagテクノロジーが拓く様々な蛍光イメージングの可能性
バイオテクノロジージャーナル, 6, 2006
永井健治
組織・個体レベルでの機能イメージングに向けて
細胞工学, 25, 1010-1013, 2006
永井健治
今、蛍光蛋白質で何ができるか？
生化学, 78, 759-763, 2006
永井健治
生細胞内ではたらく分子を可視化する
Bionics, 3, 30-35, 2006
永井健治, 小寺一平
円順列GFP変異体を利用した機能プローブの作製法と２波長励起１波長取得共焦点イメージング
実験医学別冊：染色・バイオイメージング実験ハンドブック, 羊土社, 210-215, 2006
[ Original papers ]
Kohyama J, Tokunaga A, Fujita Y, Miyoshi H, Nagai T, Miyawaki A, Nakao K, Matsuzaki Y, Okano H
Visualization of spatiotemporal activation of Notch signaling: Live monitoring and significance in neural development
Dev. Biol., 286, 311-325, 2005
Takao K, Okamoto K, Nakagawa T, Neve RL, Nagai T, Miyawaki A, Hashikawa T, Kobayashi S, Hayashi Y
Visualization of synaptic Ca2+ /calmodulin-dependent protein kinase II activity in living neurons
J. Neurosci., 25, 3107-3112, 2005
Lin X, Varnai P, Csordas G, Balla A, Nagai T, Miyawaki A, Balla T, Hajnoczky G
Control of calcium signal propagation to the mitochondria by inositol 1,4,5-trisphosphate-binding proteins
J. Biol Chem., 280, 12820-12832, 2005
Ikeda M, Ikeda-Sagara M, Okada T, Clement P, Urade Y, Nagai T, Sugiyama T, Yoshioka T, Honda K, Inoue S
Brain oxidation is an initial process in sleep induction
Neuroscience, 130, 1029-1040, 2005
[ Reviews, Books ]
永井健治，宮脇敦史
GFPを利用した蛍光バイオセンサーの作成法と生体機能の可視化
遺伝子医学Mook別冊/分子生物学実験シリーズ 図・写真で観るタンパク質構造・機能解析実験実践ガイド, メディカル ドゥ, 173-182, 2005
Miyawaki A , Nagai T & Mizuno H
Engineering fluorescent proteins
Adv.Biochem Eng Biotechnol, 95, 1-15, 2005
Miyawaki A, Nagai T & Mizuno H
Genetic probes for calcium dynamics
Yeste et al. eds. Imaging Neurons-A Laboratory Manual-, CSHL PRESS, 2005
[ 2004~2001 Original papers ]
Okamoto K, Nagai T, Miyawaki A, Hayashi Y
Rapid and persistent modulation of actin dynamics regulates postsynaptic reorganization underlying bi-directional plasticity
Nature Neurosci, 7, 1104-1112, 2004
Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A
Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins
Proc. Natl. Acad. Sci. USA, 101, 10554-10559, 2004
Iwano M, Shiba H, Miwa T, Che F, Takayama S, Nagai T, Miyawaki A, Isogai A
Ca2+ dynamics in a pollen grain and a papilla cell during pollination of arabidopsis
Plant Physiology, 136, 3562-3571, 2004
Nagai T, Miyawaki A
A high-throughput method for development of FRET-based indicators for proteolysis
Biochem Biophys Res Commun., 319, 72-77, 2004
Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A
Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer
Biochem. J., 381, 307-312, 2004
Hasan M, Friedrich R, Larkum M, Petersen C, Euler T, Duebel M, Both M, Waters J, Bujard H, Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W
Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control
PLOS, 2, 763-775, 2004
Takemoto K, Nagai T, Miyawaki A, Miura M
Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects
J. Cell Biol., 160, 235-243, 2003
Fujii H, Nagai T, Shirasawa H, Doi J, Nishimatsu S, Takeda H, Sakai M
Anteroposterior patterning in Xenopus embryos: Egg fragment assay system revealed a synergy of dorsalizing and posteriorizing embryonic domains
Dev. Biol., 252, 15-30, 2003
Hino J, Nishimatsu S, Nagai T, Matsuo H, Kangawa K, Nohno T
Coordination of BMP-3b and cerberus is required for head formation of Xenopus embryos
Dev. Biol., 260, 138-157, 2003
Rekas A, Alattia JR, Nagai T, Miyawaki A, Ikura M
Crystal structure of Venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity
J. Biol. Chem., 277, 50573-50578, 2003
Kato T, Ishiwata M, Nagai T
Mitochondrial calcium response in human transformed lymphoblastoid cells
Life Sci., 71, 581-590, 2002
Shimozono S, Fukano T, Nagai T, Kirino Y, Mizuno H, Miyawaki A
Confocal imaging of subcellular Ca2+ concentrations using a dual-excitation ratiometric indicator based on green fluorescent protein
Science STKE, 26, 125, PL4, 2002
Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
Nature Biotechnol., 20, 87-90, 2002
Robert V, Gurlini P, Tosello V, Nagai T, Miyawaki A, DiLisa F, Pozzan T
Beat-to-beat of mitochondrial [Ca2+] in cardiac cells
EMBO J., 20, 4998-5007, 2001
Mochizuki N, Yamashita S, Kurokawa K, Ohba Y, Nagai T, Miyawaki A, Matsuda M
Spatio-temporal images of growth-factor-induced activation of Ras and Rap1
Nature, 411, 1065-1068, 2001
Kitaguchi T, Nakata K, Nagai T, Aruga J & Mikoshiba K
Xenopus Polycomblike 2 (XPcl2) controls anterior to posterior patterning of the neural tissue
Dev. Genes Evol., 211, 309-314, 2001
Nagai T, Sawano A, Park ES, Miyawaki A
Circularly permuted green fluorescent protein engineered to sense Ca2+
Proc. Natl. Acad. Sci. USA, 98, 3197-3202, 2001
[ 2004~2001 Reviews, Books ]
永井健治，宮脇敦史
GFP間FRETに基づく高性能バイオセンサーの開発
医学のあゆみ, 医歯薬出版, 210, 157-158, 2004
永井健治，宮脇敦史
GFPを使ったバイオセンサーの作成法
日本組織細胞化学会編, 学際企画, 2004
Miyawaki A, Nagai T, Mizuno H
Mechanisms of protein fluorophore formation and engineering
Curr. Opin. Chem. Biol., 7, 557-562, 2003
Nagai T, Miyawaki A
Application of green fluorescent proteins in cell biology
Cytometry Research, 13, 1-10, 2003
永井健治，宮脇敦史
運動器のダイナミクスに迫るには－蛍光蛋白質を用いたバイオイメージング
医学のあゆみ, 医歯薬出版, 205, 193-196, 2003
Miyawaki A, Mizuno H, Nagai T, Sawano A
Development of genetically encoded fluorescent indicators for calcium
Methods Enzymol., 360, 202-225, 2003
永井健治
GFPを用いた方法の新たな発展
日本組織細胞化学会編, 学際企画, 97-108, 2003
永井健治，宮脇敦史
高効率に発光構造をとるGFP変異体の開発
生物物理, 244, 305-308, 2002
[ 2000~1992 Original papers ]
Nagai T, Aruga J, Minowa O, Sugimoto T, Ohno Y, Noda T, Mikoshiba K
Zic2 regulates the kinetics of neurulation
Proc. Natl. Acad. Sci. USA, 97, 1618-1623, 2000
Kitaguchi T, Nagai T, Nakata K, Aruga J, Mikoshiba K
Zic3 is involved in the left-right specification of the Xenopus embryo
Development, 127, 4787-4795, 2000
Nakata K, Nagai T, Aruga J, Mikoshiba K
Xenopus Zic family and its role in neural and neural crest development
Mech. Dev., 75, 43-51, 1998
Aruga J, Minowa O, Yaginuma H, Kuno J, Nagai T, Noda T, Mikoshiba K
Mouse Zic1 is involved in cerebellar development
J. Neurosci., 18, 284-293, 1998
Nakata K*, Nagai T*, Aruga J, Mikoshiba K
Xenopus Zic3, a primary regulator both in neural and neural crest development
Proc. Natl. Acad. Sci. USA, 94, 11980-11985, 1997 (*: These two authors contributed equally to this study)
Nagai T, Aruga J, Takada S, Gunthe, T, Sporle R, Schughart K, Mikoshiba K
The expression of the mouse Zic1, Zic2, and Zic3 gene suggests an essential role for Zic genes in body pattern formation
Dev. Biol., 182, 299-313, 1997
Aruga J, Nagai T, Tokuyama T, Hayashizaki Y, Okazaki Y, Chapman VM, Mikoshiba K
The mouse Zic gene family. Homologues of the Drosophila pair-rule gene odd-paired
J. Biol.Chem., 271, 1043-1047, 1996
Aoki S, Yoneda I, Nagai T, Ueno N, Murakami K
Nondestructive imaging of internal structures of frog (Xenopus laevis) by shadow-projection X-ray microtomography
Jpn. J. Appl. Phys., 33, 556-558, 1994
Suzuki A, Nagai T, Nishimatsu S, Sugino H, Shibai H, Murakami K, Ueno N
Autoinduction of activin genes in early Xenopus embryos
Biochem. J., 298, 275-280, 1994
Nishimatsu S, Iwao M, Nagai T, Oda S, Suzuki A, Asashima M, Murakami K, Ueno N
A carboxyl-terminal truncated version of activin receptor mediates activin signals in early Xenopus development
FEBS Lett., 312, 169-173, 1992
[ Reviews, Books ]
永井健治，宮脇敦史
アフリカツメガエル
実験医学別冊：ポストゲノム時代の実験講座3 宮脇敦史（編）羊土社, 118-129, 2000